Endometriosis Associated-miRNome Analysis of Blood Samples: A Prospective Study

被引：6

作者：

Bendifallah, Sofiane ^{[1
,2
]}

Dabi, Yohann ^{[1
,2
]}

Suisse, Stephane ^{[3
]}

Delbos, Lea ^{[4
]}

Poilblanc, Mathieu ^{[5
]}

Descamps, Philippe ^{[4
]}

Golfier, Francois ^{[5
]}

Jornea, Ludmila ^{[6
]}

Bouteiller, Delphine ^{[7
]}

Touboul, Cyril ^{[1
,2
]}

Puchar, Anne ^{[8
]}

Darai, Emile ^{[1
,2
]}

机构：

[1] Sorbonne Univ, Hop Tenon, Dept Obstet & Reprod Med, Ctr Expert Endometriose C3E,Clinical Res Grp GRC, 4 Rue Chine,GRC6 C3E SU, F-75020 Paris, France

[2] Ctr Rech St Antoine CRSA, INSERM, UMR S 938, Canc Biol & Therapeut, F-75020 Paris, France

[3] Ziwig, 19 Rue Reboud, F-69003 Lyon, France

[4] CHU Angers, Dept Obstet & Reprod Med, Endometriosis Expert Ctr Pays Loire, F-49100 Angers, France

[5] Lyon South Univ Hosp, Lyon Civil Hosp, Steering Comm EndAURA Network, Dept Obstet & Reprod Med,Endometriosis Expert Ctr, F-69310 Pierre Benite, France

[6] Sorbonne Univ, AP HP, Hop Pitie Salpetriere,UMR 7225, Paris Brain Inst,Inst Cerveau,ICM,CNRS,Inserm,U11, F-75006 Paris, France

[7] Hop La Pitie Salpetriere, Inst Cerveau & Moelle Epiniere, Genotyping & Sequencing Core Facil, IGenSeq,ICM, 47-83 Blvd Hop, F-75013 Paris, France

[8] Sorbonne Univ, Hop Tenon, Dept Obstet & Reprod Med, 4 Rue Chine, F-75020 Paris, France

来源：

DIAGNOSTICS | 2022年 / 12卷 / 05期

关键词：

endometriosis; miRNA; NGS; bioinformatics; MICRORNA EXPRESSION; RNAS; DIAGNOSIS; MIRBASE; ISSUES; WOMEN;

D O I：

10.3390/diagnostics12051150

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of our study was to describe the bioinformatics approach to analyze miRNome with Next Generation Sequencing (NGS) of 200 plasma samples from patients with and without endometriosis. Patients were prospectively included in the ENDO-miRNA study that selected patients with pelvic pain suggestive of endometriosis. miRNA sequencing was performed using an Novaseq6000 sequencer (Illumina, San Diego, CA, USA). Small RNA-seq of 200 plasma samples yielded -4228 M raw sequencing reads. A total of 2633 miRNAs were found differentially expressed. Among them, 8.6% (n = 229) were up- or downregulated. For these 229 miRNAs, the F1-score, sensitivity, specificity, and AUC ranged from 0-88.2%, 0-99.4%, 4.3-100%, and 41.5-68%, respectively. Utilizing the combined bioinformatic and NGS approach, a specific and broad panel of miRNAs was detected as being potentially suitable for building a blood signature of endometriosis.

引用

页数：11

共 66 条

[61] Circulating MicroRNAs Identified in a Genome-Wide Serum MicroRNA Expression Analysis as Noninvasive Biomarkers for Endometriosis [J].