Involvement of CPI-17 downregulation in the dysmotility of the colon from dextran sodium sulphate-induced experimental colitis in a mouse model

被引:31
|
作者
Sato, K.
Ohkura, S.
Kitahara, Y.
Ohama, T.
Hori, M.
Sato, M.
Kobayashi, S.
Sasaki, Y.
Hayashi, T.
Nasu, T.
Ozaki, H.
机构
[1] Yamaguchi Univ, Fac Agr, Dept Vet Pharmacol, Yamaguchi 7538515, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Pharmacol, Tokyo 113, Japan
[3] Yamaguchi Univ, Sch Med, Dept Mol Physiol, Ube, Yamaguchi 755, Japan
[4] Yamaguchi Univ, Fac Agr, Dept Vet Pathol, Ube, Yamaguchi 755, Japan
来源
NEUROGASTROENTEROLOGY AND MOTILITY | 2007年 / 19卷 / 06期
关键词
Ca2+-sensitization; colonic smooth muscle; CPI-17; interleukin-1; beta; motility disorder; ulcerative colitis;
D O I
10.1111/j.1365-2982.2007.00911.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The mechanism of gastrointestinal dysmotility in inflammatory bowel disease has not been clarified. In this study, we examined the mechanism involved in the inflamed distal colon isolated from a mouse model of dextran sodium sulphate-induced ulcerative colitis (DSS-treated mouse). Although substance P-induced contraction was not changed, carbachol-induced contraction was reduced in the DSS-treated mouse colon. Pre-incubation with the NO synthase inhibitor N-G-monomethyl-L-arginine (L-NMMA) or the cyclooxygenase inhibitor indomethacin did not reverse the carbachol-induced contraction in the DSS-treated mouse colon. In semi-quantitative reverse transcription-polymerase chain reaction experiments and Western blot analysis, muscarinic M-3 receptor expressions were not changed. The Ca2+-sensitization of contractile elements induced by carbachol with GTP or GTP gamma S was reduced in the beta-escin-permeabilized DSS-treated mouse colon. Although the expression of proteins such as rhoA, ROCK1, ROCK2 or MYPT1 in smooth muscles was not changed, the expression of CPI-17, the functional protein involved in smooth muscle Ca2+-sensitization, was significantly decreased in the DSS-treated mouse colon. These results suggest that the suppression of carbachol-induced contraction in mice with colitis is attributable at least partially to the increased activity of myosin phosphatase following the downregulation of CPI-17.
引用
收藏
页码:504 / 514
页数:11
相关论文
共 50 条
  • [41] Experimental colitis induced by dextran sulphate sodium in mice: beneficial effects of sulphasalazine and olsalazine
    Axelsson, LG
    Landstrom, E
    Bylund-Fellenius, AC
    ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 1998, 12 (09) : 925 - 934
  • [42] Preventive effects of cranberry products on experimental colitis induced by dextran sulphate sodium in mice
    Xiao, Xiao
    Kim, Jonggun
    Sun, Quancai
    Kim, Daeyoung
    Park, Cheon-Seok
    Lu, Tzong-Shi
    Park, Yeonhwa
    FOOD CHEMISTRY, 2015, 167 : 438 - 446
  • [43] Induction of experimental colitis by dextran sulphate sodium in a piglet model of irritable bowel syndrome
    Lackeyram, Dale
    Kim, Connie
    Young, Denise
    Archbold, Tania
    Yang, Chengbo
    Mine, Yoshinori
    Fan, Ming
    FASEB JOURNAL, 2008, 22
  • [44] Secreted Protein Acidic and Rich in Cysteine (SPARC) Exacerbates Colonic Inflammatory Symptoms in Dextran Sodium Sulphate-Induced Murine Colitis
    Ng, Yoke-Leng
    Klopcic, Borut
    Lloyd, Frances
    Forrest, Cynthia
    Greene, Wayne
    Lawrance, Ian C.
    PLOS ONE, 2013, 8 (10):
  • [45] G protein-coupled receptor kinase 6 controls chronicity and severity of dextran sodium sulphate-induced colitis in mice
    Eijkelkamp, Niels
    Heijnen, Cobi J.
    Lucas, Ayscha
    Premont, Richard T.
    Elsenbruch, Sigrid
    Schedlowski, Manfred
    Kavelaars, Annemieke
    GUT, 2007, 56 (06) : 847 - 854
  • [46] Histological and ultrastructural changes of the colon in dextran sodium sulfate-induced mouse colitis
    Xu, Xiaojuan
    Lin, Sisi
    Yang, Yanhua
    Gong, Xiaohui
    Tong, Jianhua
    Li, Kun
    Li, Yongyu
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 20 (03) : 1987 - 1994
  • [47] Preventive effects of rice bran oil on 1,2-dimethylhydrazine/dextran sodium sulphate-induced colon carcinogenesis in rats
    Shih, Chun-Kuang
    Ho, Chia-Jung
    Li, Sing-Chung
    Yang, Shwu-Huey
    Hou, Wen-Chi
    Cheng, Hsing-Hsien
    FOOD CHEMISTRY, 2011, 126 (02) : 562 - 567
  • [48] Dextran Sodium Sulphate-Induced Gastrointestinal Injury Further Aggravates the Impact of Galantamine on the Gastric Myoelectric Activity in Experimental Pigs
    Bures, Jan
    Tacheci, Ilja
    Kvetina, Jaroslav
    Radochova, Vera
    Kohoutova, Darina
    Valis, Martin
    Rejchrt, Stanislav
    Knoblochova, Veronika
    Zdarova Karasova, Jana
    PHARMACEUTICALS, 2021, 14 (06)
  • [49] Effects of Guchang Capsule on Dextran Sulphate Sodium-Induced Experimental Ulcerative Colitis in Mice
    Liu, Baoshan
    Liu, Tong
    Wang, Xiaohong
    Zheng, Xin
    Wang, Hong
    Ma, Lin
    EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2016, 2016
  • [50] Flaxseed lignan secoisolariciresinol diglucoside ameliorates experimental colitis induced by dextran sulphate sodium in mice
    Xu, Jiqu
    Tian, Guangjing
    Ma, Congcong
    Gao, Hui
    Chen, Chang
    Yang, Wei
    Deng, Qianchun
    Huang, Qingde
    Ma, Zhonghua
    Huang, Fenghong
    JOURNAL OF FUNCTIONAL FOODS, 2016, 26 : 187 - 195