Determination of volatile organic compounds in headspace above saliva specimens using SPME-GC/MS technique

被引：0

作者：

Milanowski, Maciej ^{[1
]}

Rudnicka, Joanna ^{[1
]}

Ligor, Tomasz ^{[1
]}

Buszewski, Boguslaw ^{[1
]}

机构：

[1] Nicolaus Copernicus Univ Torun, Fac Chem, Dept Environm Chem & Bloanalyt, Gagarina 7, PL-87100 Torun, Poland

来源：

PROCEEDINGS OF THE 14TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY | 2018年

关键词：

HS-SPME-GC/MS; preconcentration; saliva; volatile organic compounds;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The aim of this study was to apply headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS) to evaluate profiles of volatile organic compounds from saliva of nonsmokers and smokers. Also different types of SPME fibres were used and influence of such factors as sample volume, incubation, and adsorption time were evaluated. We found that 75 mu m Carboxen/PDMS fibre gave the highest abundances and the greatest number of extracted volatile organic compounds from saliva. Volume of 2 mL and incubation time 20 min were chosen as the best parameters of conducted experiments. In the typical profile of salivary headspace we found at least ten compounds such as: acetaldehyde, propanal, 2,3-butanedione, ethyl ether, dimethyl sulphide, and pyrrole. Daily variations of salivary constituents were investigated. The great diversity of compounds were observed in the afternoon. Comparison of smokers and nonsmokers revealed the presence of pyridine in salivary samples of persons smoking cigarettes.

引用

页码：1 / 6

页数：6

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