Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry

被引：40

作者：

Tolun, Adviye A. ^{[1
]}

Zhang, Haoyue ^{[1
]}

Il'yasova, Dora ^{[2
]}

Sztaray, Judit ^{[1
]}

Young, Sarah P. ^{[1
]}

Millington, David S. ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Div Med Genet, Dept Pediat, Durham, NC 27709 USA

[2] Duke Univ, Med Ctr, Canc Control & Prevent Program, Dept Community & Family Med, Durham, NC 27710 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 402卷 / 02期

关键词：

OXIDANT-ANTIOXIDANT BALANCE; CHRONIC LUNG-DISEASE; OXIDATIVE STRESS; LC-MS/MS; ACID; PLASMA; INDICATOR; PRODUCTS; MARKER;

D O I：

10.1016/j.ab.2010.03.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error = 6%), precise (Ultra- and interday imprecision <8%). and sensitive (limit of detection = 0.06 pmol). Allantoin levels measured in control samples were comparable to literature values. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：191 / 193

页数：3

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