Identification and characterization of CcCTR1, a copper uptake transporter-like gene, in Coprinopsis cinerea

被引：7
|
作者：
Nakagawa, Yuko [1 ]
Kikuchi, Sayaka [1 ]
Sakamoto, Yuichi [1 ]
Yano, Akira [1 ]
机构：
[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan
来源：
MICROBIOLOGICAL RESEARCH | 2010年 / 165卷 / 04期
关键词：
Copper; Transporter; Basidiomycetes; Coprinopsis cinerea; SACCHAROMYCES-CEREVISIAE; PLEUROTUS-OSTREATUS; TRAMETES-VERSICOLOR; FUNCTIONAL EXPRESSION; TRANSCRIPTION FACTORS; LENTINULA-EDODES; LACCASE ACTIVITY; YEAST; TRAFFICKING; PATHWAY;
D O I：
10.1016/j.micres.2009.05.004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Copper (Cu) is an essential element for the physiological function of organisms. In basidiomycetes, Cu is necessary for the production of phenol oxidase enzymes such as laccase and tyrosinase. We isolated and characterized two genes, CcCTR1 and -2, from the model basidionnycete Coprinopsis cinerea. CcCTR1 and -2 showed similarity to the Cu transporter CTR1 in Saccharomyces cerevisiae. Both CcCTRs had a MLxxM motif that is conserved in other CTR homologs. The addition of Cu to a liquid culture of C. cinerea decreased the mRNA accumulation of CcCTR1 and -2. Heterologous expression of CcCTR1 in S. cerevisiae increased Cu sensitivity, suggesting that CcCTR1 is a Cu uptake transporter. Together, these results suggest that CcCTR1 plays an important role in Cu accumulation in C. cinerea. (c) 2009 Elsevier GmbH. All rights reserved.
引用
收藏
页码：276 / 287
页数：12
相关论文
共 50 条
  • [31] Identification of the PA1113 Gene Product as an ABC Transporter Involved in the Uptake of Carbenicillin in Pseudomonas aeruginosa PAO1
    Hulen, Christian
    Racine, Pierre-jean
    Chevalier, Sylvie
    Feuilloley, Marc
    LOMRI, Nour-Eddine
    ANTIBIOTICS-BASEL, 2020, 9 (09): : 1 - 17
  • [32] Identification of the Mutations in the Prostaglandin Transporter Gene, SLCO2A1 and Clinical Characterization in Korean Patients with Pachydermoperiostosis
    Lee, Sihoon
    Park, So Young
    Kwon, Hyun Jin
    Lee, Chul-Ho
    Kim, Ok-Hwa
    Rhee, Yumie
    JOURNAL OF KOREAN MEDICAL SCIENCE, 2016, 31 (05) : 735 - 742
  • [33] Genome-Wide Identification, Cloning and Functional Analysis of the Zinc/Iron-Regulated Transporter-Like Protein (ZIP) Gene Family in Trifoliate Orange (Poncirus trifoliata L. Raf.)
    Fu, Xing-Zheng
    Zhou, Xue
    Xing, Fei
    Ling, Li-Li
    Chun, Chang-Pin
    Cao, Li
    Aarts, Mark G. M.
    Peng, Liang-Zhi
    FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [34] KCNE1-like gene is deleted in AMME contiguous gene syndrome: Identification and characterization of the human and mouse homologs
    Piccini, M
    Vitelli, F
    Seri, M
    Galietta, LJV
    Moran, O
    Bulfone, A
    Banfi, S
    Pober, B
    Renieri, A
    GENOMICS, 1999, 60 (03) : 251 - 257
  • [35] Genome-Wide Identification and Characterization of the Polyamine Uptake Transporter (Put) Gene Family in Tomatoes and the Role of Put2 in Response to Salt Stress
    Zhong, Min
    Yue, Lingqi
    Liu, Wei
    Qin, Hongyi
    Lei, Bingfu
    Huang, Riming
    Yang, Xian
    Kang, Yunyan
    ANTIOXIDANTS, 2023, 12 (02)
  • [36] The fission yeast copper-sensing transcription factor Cuf1 regulates the copper transporter gene expression through an Ace1/Amt1-like recognition sequence
    Beaudoin, J
    Labbé, S
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (18) : 15472 - 15480
  • [37] Identification and functional characterization of Polo-like kinase 1, a novel gene responsible for progression of cutaneous melanoma
    Moser, A.
    Pashenkov, M.
    Jalili, A.
    Wagner, C.
    Stingl, G.
    Ramaswamy, S.
    Brunet, J.
    Golub, T. R.
    Wagner, S.
    EXPERIMENTAL DERMATOLOGY, 2008, 17 (03) : 277 - 277
  • [38] Characterization of class 1 integron resistance gene cassettes and the identification of a novel IS-like element in Acinetobacter baumannii
    Segal, H
    Thomas, R
    Elisha, BG
    PLASMID, 2003, 49 (02) : 169 - 178
  • [39] KCNE1 like gene id deleted in AMME contiguous gene syndrome: identification and characterization of the human and mouse homologs.
    Renieri, A
    Vitelli, F
    Piccini, M
    Meloni, I
    Galietta, LJ
    Moran, O
    Bulfone, A
    Banfi, S
    Pober, B
    AMERICAN JOURNAL OF HUMAN GENETICS, 1999, 65 (04) : A111 - A111
  • [40] Identification of six putative anion transporter genes in human and characterization of PAT1 a candidate gene for pancreatic anion exchanger.
    Lohi, H
    Kujala, M
    Kerkela, E
    Saarialho-Kere, U
    Kestila, M
    Kere, J
    AMERICAN JOURNAL OF HUMAN GENETICS, 2000, 67 (04) : 176 - 176
12345