Identification and characterization of CcCTR1, a copper uptake transporter-like gene, in Coprinopsis cinerea

被引：7
|
作者：
Nakagawa, Yuko [1 ]
Kikuchi, Sayaka [1 ]
Sakamoto, Yuichi [1 ]
Yano, Akira [1 ]
机构：
[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan
来源：
MICROBIOLOGICAL RESEARCH | 2010年 / 165卷 / 04期
关键词：
Copper; Transporter; Basidiomycetes; Coprinopsis cinerea; SACCHAROMYCES-CEREVISIAE; PLEUROTUS-OSTREATUS; TRAMETES-VERSICOLOR; FUNCTIONAL EXPRESSION; TRANSCRIPTION FACTORS; LENTINULA-EDODES; LACCASE ACTIVITY; YEAST; TRAFFICKING; PATHWAY;
D O I：
10.1016/j.micres.2009.05.004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Copper (Cu) is an essential element for the physiological function of organisms. In basidiomycetes, Cu is necessary for the production of phenol oxidase enzymes such as laccase and tyrosinase. We isolated and characterized two genes, CcCTR1 and -2, from the model basidionnycete Coprinopsis cinerea. CcCTR1 and -2 showed similarity to the Cu transporter CTR1 in Saccharomyces cerevisiae. Both CcCTRs had a MLxxM motif that is conserved in other CTR homologs. The addition of Cu to a liquid culture of C. cinerea decreased the mRNA accumulation of CcCTR1 and -2. Heterologous expression of CcCTR1 in S. cerevisiae increased Cu sensitivity, suggesting that CcCTR1 is a Cu uptake transporter. Together, these results suggest that CcCTR1 plays an important role in Cu accumulation in C. cinerea. (c) 2009 Elsevier GmbH. All rights reserved.
引用
收藏
页码：276 / 287
页数：12
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