Ten years of R&D and full automation in molecular diagnosis

被引：69
作者：
Greub, Gilbert [1 ,2 ]
Sahli, Roland [1 ,2 ]
Brouillet, Rene [1 ,2 ]
Jaton, Katia [1 ,2 ]
机构：
[1] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
[2] Univ Hosp Ctr, Lausanne, Switzerland
来源：
FUTURE MICROBIOLOGY | 2016年 / 11卷 / 03期
关键词：
assay development; automation; flexibility; high-throughput tests; intracellular bacteria; molecular diagnosis; quality assessment; real-time PCR; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; STEM-CELL TRANSPLANTATION; HERPES-SIMPLEX-VIRUS; POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDER; CEREBROSPINAL-FLUID PCR; RIBOSOMAL-RNA GENE; RAPID DETECTION; CHLAMYDIA-PNEUMONIAE; MYCOBACTERIUM-TUBERCULOSIS;
D O I：
10.2217/fmb.15.152
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A 10-year experience of our automated molecular diagnostic platform that carries out 91 different real-time PCR is described. Progresses and future perspectives in molecular diagnostic microbiology are reviewed: why automation is important; how our platform was implemented; how homemade PCRs were developed; the advantages/disadvantages of homemade PCRs, including the critical aspects of troubleshooting and the need to further reduce the turnaround time for specific samples, at least for defined clinical settings such as emergencies. The future of molecular diagnosis depends on automation, and in a novel perspective, it is time now to fully acknowledge the true contribution of molecular diagnostic and to reconsider the indication for PCR, by also using these tests as first-line assays.
引用
收藏
页码：403 / 425
页数：23
相关论文
共 95 条
  • [31] Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections
    Gadsby, N. J.
    McHugh, M. P.
    Russell, C. D.
    Mark, H.
    Morris, A. Conway
    Laurenson, I. F.
    Hill, A. T.
    Templeton, K. E.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2015, 21 (08) : 788.e1 - 788.e13
  • [32] Adenoviral load diagnostics by quantitative polymerase chain reaction: techniques and application
    Ganzenmueller, Tina
    Heim, Albert
    [J]. REVIEWS IN MEDICAL VIROLOGY, 2012, 22 (03) : 194 - 208
  • [33] Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples
    Giulieri, Stefano
    Jaton, Katia
    Cometta, Alain
    Trellu, Laurence T.
    Greub, Gilbert
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2012, 64 (01): : 92 - 97
  • [34] Outbreak of Mycobacterium haemophilum Infections after Permanent Makeup of the Eyebrows
    Giulieri, Stefano
    Morisod, Benoit
    Edney, Timothy
    Oedman, Micaela
    Genne, Daniel
    Malinverni, Raffaele
    Hammann, Catherine
    Musumeci, Enrico
    Voide, Cathy
    Greub, Gilbert
    Masserey, Eric
    Bille, Jacques
    Cavassini, Matthias
    Jaton, Katia
    [J]. CLINICAL INFECTIOUS DISEASES, 2011, 52 (04) : 488 - 491
  • [35] Rapid detection of enterovirus in cerebrospinal fluid by a fully-automated PCR assay is associated with improved management of aseptic meningitis in adult patients
    Giulieri, Stefano G.
    Chapuis-Taillard, Caroline
    Manuel, Oriol
    Hugli, Olivier
    Pinget, Christophe
    Wasserfallen, Jean-Blaise
    Sahli, Roland
    Jaton, Katia
    Marchetti, Oscar
    Meylan, Pascal
    [J]. JOURNAL OF CLINICAL VIROLOGY, 2015, 62 : 58 - 62
  • [36] Etiology of Pericarditis in a Prospective Cohort of 1162 Cases
    Gouriet, Frederique
    Levy, Pierre-Yves
    Casalta, Jean-Paul
    Zandotti, Christine
    Collart, Frederic
    Lepidi, Hubert
    Cautela, Jennifer
    Bonnet, Jean Louis
    Thuny, Franck
    Habib, Gilbert
    Raoult, Didier
    [J]. AMERICAN JOURNAL OF MEDICINE, 2015, 128 (07) : 784.e1 - 784.e8
  • [37] Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples
    Goy, G.
    Croxatto, A.
    Posfay-Barbe, K. M.
    Gervaix, A.
    Greub, G.
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2009, 28 (12) : 1483 - 1486
  • [38] Diagnosis of infectious endocarditis in patients undergoing valve surgery
    Greub, G
    Lepidi, H
    Rovery, C
    Casalta, JP
    Habib, G
    Collard, F
    Fournier, PE
    Raoult, D
    [J]. AMERICAN JOURNAL OF MEDICINE, 2005, 118 (03) : 230 - 238
  • [39] Practical experience of high throughput real time PCR in the routine diagnostic virology setting
    Gunson, R. N.
    Collins, T. C.
    Carman, W. F.
    [J]. JOURNAL OF CLINICAL VIROLOGY, 2006, 35 (04) : 355 - 367
  • [40] Multicenter Evaluation of the BD Max Enteric Bacterial Panel PCR Assay for Rapid Detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga Toxin 1 and 2 Genes
    Harrington, S. M.
    Buchan, B. W.
    Doern, C.
    Fader, R.
    Ferraro, M. J.
    Pillai, D. R.
    Rychert, J.
    Doyle, L.
    Lainesse, A.
    Karchmer, T.
    Mortensen, J. E.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (05) : 1639 - 1647
12345678910