Ten years of R&D and full automation in molecular diagnosis

被引：70

作者：

Greub, Gilbert ^{[1
,2
]}

Sahli, Roland ^{[1
,2
]}

Brouillet, Rene ^{[1
,2
]}

Jaton, Katia ^{[1
,2
]}

机构：

[1] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland

[2] Univ Hosp Ctr, Lausanne, Switzerland

来源：

FUTURE MICROBIOLOGY | 2016年 / 11卷 / 03期

关键词：

assay development; automation; flexibility; high-throughput tests; intracellular bacteria; molecular diagnosis; quality assessment; real-time PCR; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; STEM-CELL TRANSPLANTATION; HERPES-SIMPLEX-VIRUS; POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDER; CEREBROSPINAL-FLUID PCR; RIBOSOMAL-RNA GENE; RAPID DETECTION; CHLAMYDIA-PNEUMONIAE; MYCOBACTERIUM-TUBERCULOSIS;

D O I：

10.2217/fmb.15.152

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 10-year experience of our automated molecular diagnostic platform that carries out 91 different real-time PCR is described. Progresses and future perspectives in molecular diagnostic microbiology are reviewed: why automation is important; how our platform was implemented; how homemade PCRs were developed; the advantages/disadvantages of homemade PCRs, including the critical aspects of troubleshooting and the need to further reduce the turnaround time for specific samples, at least for defined clinical settings such as emergencies. The future of molecular diagnosis depends on automation, and in a novel perspective, it is time now to fully acknowledge the true contribution of molecular diagnostic and to reconsider the indication for PCR, by also using these tests as first-line assays.

引用

页码：403 / 425

页数：23

共 95 条

[1]

[Anonymous], 2011, WHO GUID APPR GUID R

[2] Role of real-time reverse transcription polymerase chain reaction for detection of respiratory viruses in critically ill children with respiratory disease: Is it time for a change in algorithm? [J].