Fluorescence enhancement monitoring of pyrromethene laser dyes by metallic Ag nanoparticles

Fluorescence enhancement monitoring of pyrromethene laser dyes using their complexation with Ag nanoparticles ( Ag NPs) was studied. The size of the prepared Ag NPs was determined by transmission electron spectroscopy and UV/Vis absorption spectroscopy. Mie theory was also used to confirm the size of NPs theoretically. The effect of different nanoparticle concentrations on the optical properties of 1 x 10(-4) M PM dyes shows that 40% of Ag NPs concentration (40% C Ag NPs) in complex is the optimum concentration. Also, the effects of different concentrations of PM dyes in a complex was measured. Emission enhancement factors were calculated for all samples. Fluorescence enhancement efficiencies depended on the input pumping energy of a Nd-YAG laser (wavelength 532 nm and 8 ns pulse duration) were reported and showed the lowest energy (28 and 32 mJ) in the case of PM567 and PM597, respectively. Copyright (C) 2014 John Wiley & Sons, Ltd.