Superoxide production and antioxidant enzymes in ammonia intoxication in rats

被引:186
|
作者
Kosenko, E [1 ]
Kaminsky, Y [1 ]
Kaminsky, A [1 ]
Valencia, M [1 ]
Lee, L [1 ]
Hermenegildo, C [1 ]
Felipo, V [1 ]
机构
[1] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142292, Russia
关键词
hyperammonemia; superoxide radical; antioxidants enzymes; brain; ammonia toxicity; free radicals;
D O I
10.3109/10715769709097867
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Injection of large doses of ammonium salts lead to the rapid death of animals. However, the molecular mechanisms involved in ammonia toxicity remain to be clarified. We have tested the effect of injecting 7 mmol/kg of ammonium acetate on the production of superoxide and on the activities of some antioxidant enzymes in rat liver, brain, erythrocytes and plasma. Glutathione peroxidase, superoxide dismutase and catalase activities were decreased in liver and brain (both in cytosolic and mitochondrial fractions) and also in blood red cells, while glutathione reductase activity remained unchanged. Superoxide production in submitochondrial particles from liver and brain was increased by more than 100% in both tissues. Both diminished activity of antioxidant enzymes and increased superoxide radical production could lead to oxidative stress and cell damage, which could be involved in the mechanism of acute ammonia toxicity.
引用
收藏
页码:637 / 644
页数:8
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