Optimisation of Xylanase-Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

被引：13

作者：

Nawawi, Muhammad Hariadi ^{[1
]}

Ismail, Khairul Izdihar ^{[1
]}

Sa'ad, Norazliza ^{[1
]}

Mohamad, Rosfarizan ^{[2
]}

Tahir, Paridah Md ^{[3
]}

Asa'ari, Ainun Zuriyati ^{[3
]}

Saad, Wan Zuhainis ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Serdang 43400, Malaysia

[2] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Serdang 43400, Malaysia

[3] Univ Putra Malaysia, Inst Trop Forests & Forestry Prod, Serdang 43400, Malaysia

来源：

FERMENTATION-BASEL | 2022年 / 8卷 / 03期

关键词：

RSM; xylanase; pectinase; Bacillus; production; SOLID-STATE FERMENTATION; CELLULASE-FREE XYLANASE; ASPERGILLUS-NIGER; AGRICULTURAL WASTES; PROTEASE PRODUCTION; PHENOL DEGRADATION; BACTERIAL ISOLATE; ALPHA-AMYLASE; SUBTILIS; ENZYMES;

D O I：

10.3390/fermentation8030119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 degrees C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model's validity. The optimised CCD model had a 1.34-fold, 159 +/- 6 U/mL greater xylanase and 5.96-fold, 205 +/- 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase-pectinase resulted in a ratio of 1:1.3.

引用

页数：17

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