Optimisation of Xylanase-Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

被引：14

作者：

Nawawi, Muhammad Hariadi ^{[1
]}

Ismail, Khairul Izdihar ^{[1
]}

Sa'ad, Norazliza ^{[1
]}

Mohamad, Rosfarizan ^{[2
]}

Tahir, Paridah Md ^{[3
]}

Asa'ari, Ainun Zuriyati ^{[3
]}

Saad, Wan Zuhainis ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Serdang 43400, Malaysia

[2] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Serdang 43400, Malaysia

[3] Univ Putra Malaysia, Inst Trop Forests & Forestry Prod, Serdang 43400, Malaysia

来源：

FERMENTATION-BASEL | 2022年 / 8卷 / 03期

关键词：

RSM; xylanase; pectinase; Bacillus; production; SOLID-STATE FERMENTATION; CELLULASE-FREE XYLANASE; ASPERGILLUS-NIGER; AGRICULTURAL WASTES; PROTEASE PRODUCTION; PHENOL DEGRADATION; BACTERIAL ISOLATE; ALPHA-AMYLASE; SUBTILIS; ENZYMES;

D O I：

10.3390/fermentation8030119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 degrees C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model's validity. The optimised CCD model had a 1.34-fold, 159 +/- 6 U/mL greater xylanase and 5.96-fold, 205 +/- 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase-pectinase resulted in a ratio of 1:1.3.

引用

页数：17

共 80 条

[1] Enhanced production of bacterial xylanase and its utility in saccharification of sugarcane bagasse [J].

Alokika ;

Singh, Bijender .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2020, 43 (06) :1081-1091

[2]

Annamalai N, 2009, INDIAN J BIOTECHNOL, V8, P291

[3] Comparative characterization of commercially important xylanase enzymes [J].

Arora, Neelima ;

Banerjee, Amit Kumar ;

Mutyala, Srilaxmi ;

Murty, Upadhyayula Suryanarayana .

BIOINFORMATION, 2009, 3 (10) :446-453

[4]

Azzaz H. H., 2012, Research Journal of Microbiology, V7, P23, DOI 10.3923/jm.2012.23.31

[5] Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice [J].

Barman, Sumi ;

Sit, Nandan ;

Badwaik, Laxmikant S. ;

Deka, Sankar C. .

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2015, 52 (06) :3579-3589

[6]

Begam A, 2015, Int adv res j, V2, DOI [10.17148/IARJSET.2015.2907, DOI 10.17148/IARJSET.2015.2907]

[7] Statistical Optimization of Pectinase Biosynthesis from Orange Peel by Bacillus licheniformis Using Submerged Fermentation [J].

Bibi, Nowshad ;

Ali, Sikander ;

Tabassum, Romana .

WASTE AND BIOMASS VALORIZATION, 2016, 7 (03) :467-481

[8] Kinetic study of the thermal denaturation of a hyperthermostable extracellular α-amylase from Pyrococcus furiosus [J].

Brown, I. ;

Dafforn, T. R. ;

Fryer, P. J. ;

Cox, P. W. .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2013, 1834 (12) :2600-2605

[9] Production of cellulase in solid-state fermentation with Trichoderma reesei MCG 80 on wheat straw [J].

Chahal, PS ;

Chahal, DS ;

Le, GBB .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1996, 57-8 :433-442

[10] Sequential solid-state and submerged cultivation of Aspergillus niger on sugarcane bagasse for the production of cellulase [J].

Cunha, F. M. ;

Esperanca, M. N. ;

Zangirolami, T. C. ;

Badino, A. C. ;

Farinas, C. S. .

BIORESOURCE TECHNOLOGY, 2012, 112 :270-274

← 1 2 3 4 5 6 7 8 →