Cytotoxicity Effects of Graphene and Single-Wall Carbon Nanotubes in Neural Phaeochromocytoma-Derived PC12 Cells

被引：981

作者：

Zhang, Yongbin ^{[1
]}

Ali, Syed F. ^{[1
]}

Dervishi, Enkeleda ^{[2
]}

Xu, Yang ^{[2
]}

Li, Zhongrui ^{[2
]}

Casciano, Daniel ^{[2
]}

Biris, Alexandru S. ^{[2
]}

机构：

[1] US FDA, Neurochem Lab, Div Neurotoxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

[2] Univ Arkansas, Nanotechnol Ctr, Dept Appl Sci, Little Rock, AR 72204 USA

来源：

ACS NANO | 2010年 / 4卷 / 06期

关键词：

graphene; single-wall carbon nanotubes; cytotoxicity; shape; PC12; cells; reactive oxidative species; PULMONARY TOXICITY;

D O I：

10.1021/nn1007176

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphitic nanomaterials such as graphene layers (G) and single-wall carbon nanotubes (SWCNT) are potential candidates in a large number of biomedical applications. However, little is known about the effects of these nanomaterials on biological systems. Here we show that the shape of these materials is directly related to their induced cellular toxicity. Both G and SWCNT induce cytotoxic effects, and these effects are concentration-and shape-dependent. Interestingly, at low concentrations, G induced stronger metabolic activity than SWCNT, a trend that reversed at higher concentrations. Lactate dehydrogenase levels were found to be significantly higher for SWCNT as compared to the G samples. Moreover, reactive oxygen species were generated in a concentration-and time-dependent manner after exposure to G, indicating an oxidative stress mechanism. Furthermore, time-dependent caspase 3 activation after exposure to G (10 mu g/mL) shows evidence of apoptosis. Altogether these studies suggest different biological activities of the graphitic nanomaterials, with the shape playing a primary role.

引用

页码：3181 / 3186

页数：6

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