Perfluorocarbon emulsions cytotoxic effects on human fibroblasts and effect of aging on particle size distribution

被引：9

作者：

Centis, Valerie

Doillon, Charles J.

Vermette, Patrick

机构：

[1] Univ Sherbrooke, Dept Chem Engn, Lab Bioingn & Biophys, Sherbrooke, PQ J1K 2R1, Canada

[2] Univ Laval, Oncol & Mol Endocrinol Res Ctr, CHU Laval, Res Ctr, Quebec City, PQ, Canada

[3] Univ Laval, Dept Surg, Fac Med, Quebec City, PQ, Canada

[4] Univ Sherbrooke, Inst Geriatrie, Res Ctr Aging, Sherbrooke, PQ J1K 2R1, Canada

来源：

ARTIFICIAL ORGANS | 2007年 / 31卷 / 08期

关键词：

perfluorocarbon emulsions; perfluorooctyl bromide; oxygen carriers; cytotoxicity; fibroblasts; cell morphology; cytocompatibility;

D O I：

10.1111/j.1525-1594.2007.00437.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The purpose of this study was to characterize emulsion preparations made of perfluorooctyl bromide (PFOB) and egg yolk phospholipid (EYP) and their cytotoxicity. Dynamic light scattering and transmission electron microscopy revealed that freshly prepared emulsions stored at different temperatures for a 24-h period have a unimodal particle size distribution with an average particle size of ca. 200 nm. The emulsion displayed a broader particle size distribution following a 14-day storage. Primary human fibroblasts exposure to PFOB/EYP emulsions permanently inhibited cell proliferation and decreased mitochondrial activity. Scanning electron microscopy pictures reveal the presence of spherical particles on the fibroblasts following exposure to the emulsions after thorough rinsing with culture media.

引用

页码：649 / 653

页数：5

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