Benzo(a)Pyrene Causes PRKAA1/2-Dependent ID2 Loss in Trophoblast Stem Cells

被引:32
作者
Xie, Yufen [1 ,2 ]
Abdallah, Mazen E. [1 ,2 ]
Awonuga, Awoniyi O. [1 ,2 ]
Slater, Jill A. [3 ,4 ]
Puscheck, Elizabeth E. [1 ,2 ]
Rappolee, Dan A. [1 ,2 ,3 ,4 ,5 ,6 ,7 ]
机构
[1] Wayne State Univ, Sch Med, CS Mott Ctr Human Growth & Dev, Detroit, MI 48201 USA
[2] Wayne State Univ, Sch Med, Dept Ob Gyn Reprod Endocrinol & Infertil, Detroit, MI 48201 USA
[3] Wayne State Univ, Sch Med, Reprod Sci Program, Detroit, MI 48201 USA
[4] Wayne State Univ, Sch Med, Dept Physiol, Detroit, MI 48201 USA
[5] Wayne State Univ, Sch Med, Inst Environm Hlth & Safety, Detroit, MI 48201 USA
[6] Wayne State Univ, Sch Med, Karmanos Canc Inst, Detroit, MI 48201 USA
[7] Univ Windsor, Dept Biol, Windsor, ON N9B 3P4, Canada
关键词
ACTIVATED PROTEIN-KINASE; HUMAN CYTOTROPHOBLAST DIFFERENTIATION; PREIMPLANTATION MOUSE EMBRYOS; HELIX TRANSCRIPTION FACTOR; HUMAN-PLACENTA; MATERNAL SMOKING; IN-VITRO; HYPEROSMOLAR STRESS; JEG-3; CELLS; PROLIFERATION;
D O I
10.1002/mrd.21178
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Benzo(a)pyrene (BaP), a cigarette smoke component, is metabolized to diol esters (BPDE) that bind to DNA and form mutagenic BPDE-DNA adducts. BaP activates stress enzymes including stress-activated protein kinase/jun kinase (MAPK8/9) in embryos, AMP-activated protein kinase alpha1/2 subunits (PRKAA1/2) in somatic cells, and inhibits the proliferation of trophoblast cell lineages. The loss of transcription factor inhibitor of differentiation (ID)2 is required for the initial differentiation of mouse trophoblast stem cells (TSC) in implanting mouse embryo to produce the first placental hormone, chorionic sommatomammotropin (CSH)1. Here we demonstrate that BaP activates PRKAA1/2 and causes ID2 protein loss in TSC in a time- and dose-dependent manner. Although PRKAA1/2 was activated at low BaP doses, PRKAA1/2-dependent ID2 protein loss occurred at a dose that was similar to the threshold that results in a significant decrease in TSC accumulation and decreased fraction of proliferating TSC. This suggests a possible relationship between stress-induced declines in cell accumulation and stem cell differentiation when BaP levels are high. The threshold BaP dose that induces significant ID2 loss is in the range of a 2-3 pack/day habit, suggesting that this mechanism may be involved with implantation failure in smoking women.
引用
收藏
页码:533 / 539
页数:7
相关论文
共 42 条
  • [1] Detection of benzo[a]pyrene-DNA adducts in human placenta and umbilical cord blood
    Arnould, JP
    Verhoest, P
    Bach, V
    Libert, JP
    Belegaud, J
    [J]. HUMAN & EXPERIMENTAL TOXICOLOGY, 1997, 16 (12): : 716 - 721
  • [2] Chai N, 1998, DEV BIOL, V198, P105, DOI 10.1016/S0012-1606(98)80031-6
  • [3] AMP-activated protein kinase is involved in hormone-induced mouse oocyte meiotic maturation in vitro
    Chen, Jing
    Downs, Stephen M.
    [J]. DEVELOPMENTAL BIOLOGY, 2008, 313 (01) : 47 - 57
  • [4] Transcription factors underlying the development and endocrine functions of the placenta
    Cross, JC
    Anson-Cartwright, L
    Scott, IC
    [J]. RECENT PROGRESS IN HORMONE RESEARCH, VOL 57: REPRODUCTIVE HORMONES & HUMAN HEALTH, 2002, 57 : 221 - 234
  • [5] CROSS JC, 1995, DEVELOPMENT, V121, P2513
  • [6] Nitric oxide protects human extravillous trophoblast cells from apoptosis by a cyclic GMP-dependent mechanism and independently of caspase 3 nitrosylation
    Dash, PR
    Cartwright, JE
    Baker, PN
    Johnstone, AP
    Whitley, GSJ
    [J]. EXPERIMENTAL CELL RESEARCH, 2003, 287 (02) : 314 - 324
  • [7] Benzo[a]pyrene, but not 2,3,7,8-TCDD, induces G2/M cell cycle arrest, p21CIP1 and p53 phosphorylation in human choriocarcinorna JEG-3 cells:: A distinct signaling pathway
    Drukteinis, JS
    Medrano, T
    Ablordeppey, EA
    Kitzman, JM
    Shiverick, KT
    [J]. PLACENTA, 2005, 26 : S87 - S95
  • [8] Benzo[a]pyrene-induced cell cycle progression is through ERKs/cyclin D1 pathway and requires the activation of JNKs and p38 mapk in human diploid lung fibroblasts
    Du, Hong Ju
    Tang, Ning
    Liu, Bing Ci
    You, Bao Rong
    Shen, Fu Hai
    Ye, Meng
    Gao, Ai
    Huang, Chuan shu
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2006, 287 (1-2) : 79 - 89
  • [9] QUANTITATIVE ASSOCIATIONS BETWEEN DNA DAMAGE IN HUMAN-PLACENTA AND MATERNAL SMOKING AND BIRTH-WEIGHT
    EVERSON, RB
    RANDERATH, E
    SANTELLA, RM
    AVITTS, TA
    WEINSTEIN, IB
    RANDERATH, K
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1988, 80 (08) : 567 - 576
  • [10] DEVELOPMENTAL ONSET OF MIXED-FUNCTION OXIDASE ACTIVITY IN PRE-IMPLANTATION MOUSE EMBRYOS
    FILLER, R
    LEW, KJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11): : 6991 - 6995