Inhibitory Effect of Condensed Tannins from Banana Pulp on Cholesterol Esterase and Mechanisms of Interaction

被引：36
作者：
Li, Xiangxin [1 ]
Jiang, Haitao [1 ]
Pu, Yijing [1 ]
Cao, Jiankang [1 ]
Jiang, Weibo [1 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, 17 Qnghuadonglu Rd, Beijing 100083, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 51期
关键词：
cholesterol metabolism; polyphenol; noncovalent interaction; multispectroscopy methods; thermodynamics; computer molecular simulations; ALPHA-AMYLASE ACTIVITY; PANCREATIC LIPASE; KINETIC-ANALYSIS; SERUM-ALBUMIN; BINDING; DRUGS; PROANTHOCYANIDINS; ATHEROSCLEROSIS; PROCYANIDINS; ANTIOXIDANT;
D O I：
10.1021/acs.jafc.9b05212
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
In the present study, the inhibitory effect of condensed tannins (CTs) on cholesterol esterase (CEase) was studied. The underlying mechanisms were evaluated by reaction kinetics, turbidity and particle size analyses, multispectroscopy methods, thermodynamics, and computer molecular simulations. CTs showed potent CEase inhibitory activity with an IC50 value of 64.19 mu g/mL, and the CEase activity decreased with increasing CT content in a mixed-competitive manner, which was verified by molecular docking simulations. Fluorescence and UV-vis measurements revealed that complexes were formed from CEase and CTs by noncovalent interaction. Isothermal titration calorimetry indicated that the interaction between CEase and CTs occurred through hydrogen bonding and hydrophobic interactions. Circular dichroism analysis suggested that CTs inhibited the activity of CEase by altering the secondary structure of CEase. The inhibition of CTs on CEase in the gastrointestinal tract might be one mechanism for its cholesterol-lowering effect.
引用
收藏
页码：14066 / 14073
页数：8
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