Cytoskeleton alteration in MCF7R cells, a multidrug resistant human breast cancer cell line

被引:0
作者
Bichat, F [1 ]
Mouawad, R [1 ]
SolisRecendez, G [1 ]
Khayat, D [1 ]
Bastian, G [1 ]
机构
[1] SERV ONCOL MED PITIE SALPETRIERE, DEPT MED ONCOL, F-75013 PARIS, FRANCE
关键词
intermediate filaments; breast cancer MCF7; multidrug resistance; cytoskeleton; reversal activities;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Various cytoskeleton modifications are associated with malignant cell transformation and have been used as prognostic factors. A human breast cancer cell line (MCF7S) and its multidrug resistant (MDR) subline (MCF7R) were characterized here for their intermediate filaments (IFs) expression (cytokeratin 8, 18, 19 and vimentin) as a function of their resistance phenotype. Modifications of these cytoskeleton molecules were analyzed by flow cytometry, immunofluorescence, electrophoresis and immunoblotting techniques. Cytokeratins 8 and 18 were similarly expressed in the cell lines. Cytokeratin 19 was expressed in the MCF7S cell line and not in the MCF7R variant while vimentin was highly expressed in MCF7R and slightly in MCF7S. Analysis of Ifs after the addition of doxorubicin (Dox) in the culture medium of MCF7S, showed an increase in cytokeratin 8 filaments. Vimentin expression in MCF7R was not modified in the presence of these different MDR modulators. Acquisition of MDR was associated with an increase and a redistribution of vimentin filaments characterized by a perinuclear polarization. These drug resistance associated changes might derive from different biological processes triggered by chemotherapy. In conclusion, this suggests that this intermediate filament could be a marker associated with chemoresistance or a marker of malignancy in certain epithelial cancers.
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收藏
页码:3393 / 3401
页数:9
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