Molecular detection and genetic characterization of Toxoplasma gondii in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong province, eastern China

被引：8
作者：
Zhou, Dong-Hui [1 ]
Zheng, Wen-Bin [1 ,2 ]
Hou, Jun-Ling [1 ]
Ma, Jian-Gang [1 ,2 ]
Zhang, Xiao-Xuan [1 ,2 ]
Zhu, Xing-Quan [1 ]
Cong, Wei [1 ,3 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China
[2] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin Province, Peoples R China
[3] Shandong Univ, Coll Marine Sci, Weihai 264209, Shandong, Peoples R China
来源：
ACTA TROPICA | 2017年 / 172卷
基金：
中国国家自然科学基金;
关键词：
Toxoplasma gondii; Genotype; Mn-PCR-RFLP; Raccoon dog; China; RISK-FACTORS; GENOTYPIC CHARACTERIZATION; POPULATION-STRUCTURE; ARCTIC FOXES; INFECTION; STRAINS; PCR; SEROPREVALENCE; PREVALENCE; IDENTIFICATION;
D O I：
10.1016/j.actatropica.2017.05.001
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxoplasma gondii is a successful opportunistic parasite, affecting a wide range of vertebrate animals and humans. Genetic diversity of T. gondii in raccoon dogs (Nyctereutes procyonoides) is of great importance to understand the transmission of T. gondii in the environment. However, no information is available about the distribution of genetic diversity of T. gondii infection in raccoon dogs. This study was conducted to estimate the prevalence and genetic characterization of T. gondii from raccoon dogs in Shandong province, eastern China. A total of 314 brain tissue samples of raccoon dogs were collected and genomic DNA was extracted and assayed for T. gondii infection using semi-nested PCR targeting B1 gene. The positive DNA samples were typed at 10 genetic markers (SAGI, SAG2(5' + 3' SAG2, alter.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) by multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCRRFLP) technology. Thirty-five (11.15%) of 314 DNA samples were detected positive. Only six samples were completely typed at all genetic loci, and these samples represented ToxoDB genotype#9. Two sample were typed at 9 genetic loci and one was typed at 8 genetic loci, all of them represented Type I. To our knowledge, this is the first report of genetic characterization of T. gondii in raccoon dogs in China. These results revealed the existence of genetic diversity of T. gondii in raccoon dogs in China. These data provided base-line information for controlling T. gondii infection in raccoon dogs.
引用
收藏
页码：143 / 146
页数：4
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