Amperometric biosensor based on glycerol oxidase for glycerol determination

被引：26

作者：

Goriushkina, T. B. ^{[1
,2
]}

Shkotova, L. V. ^{[1
]}

Gayda, G. Z. ^{[3
]}

Klepach, H. M. ^{[3
,4
]}

Gonchar, M. V. ^{[3
,5
]}

Soldatkin, A. P. ^{[1
]}

Dzyadevych, S. V. ^{[1
]}

机构：

[1] Natl Acad Sci Ukraine, Lab Biomol Elect, Inst Mol Biol & Genet, UA-03143 Kiev, Ukraine

[2] Natl Turas Shevchenko Univ Kyiv, Kiev, Ukraine

[3] Natl Acad Sci Ukraine, Inst Cell Biol, Lvov, Ukraine

[4] Drohobych Ivan Franko State Pedag, Drogobych, Ukraine

[5] Rzeszow Univ, Fac Biotechnol, Kolbuszowa, Poland

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 144卷 / 02期

关键词：

Amperometric biosensor; Glycerol oxidase; Glycerol; Immobilization; Electrochemical polymerization; LACTATE ANALYSIS; FERMENTATION; DEHYDROGENASE; ENZYME; WINE;

D O I：

10.1016/j.snb.2008.11.051

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A comparative analysis of the efficiency of using glycerol oxidase preparations, which differ in their characteristics and methods of production, in the development of ail amperometric biosensor for glycerol determination, was performed. The enzyme preparation which, being immobilized oil the transducer surface, ensured the best working characteristics of the sensor, was selected. Electrochemical polymerization of the selected enzyme preparation in polymer poly(3,4-ethylenedioxythiophene) was chosen as the most effective method of glycerol oxidase immobilization oil the surface of amperometric biosensor. pH optimum of the developed amperometric biosensor was determined to be 7.2. Buffer capacity and background electrolyte concentration in buffer Solution were shown to have no effect oil the work of glycerol biosensor. Glycerol concentration in wine sample solutions was measured using the developed amperometric biosensor based on glycerol oxidase. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：361 / 367

页数：7

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