Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA

被引：110
作者：
Gerber, Priscilla F. [1 ]
Gong, Qiaoling [1 ]
Huang, Yao-Wei [2 ,3 ]
Wang, Chong [1 ]
Holtkamp, Derald [1 ]
Opriessnig, Tanja [1 ,4 ,5 ]
机构：
[1] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA
[2] Zhejiang Univ, Inst Prevent Vet Med, Hangzhou 310003, Zhejiang, Peoples R China
[3] Zhejiang Univ, Key Lab Anim Virol, Minist Agr, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China
[4] Univ Edinburgh, Roslin Inst, Edinburgh, Midlothian, Scotland
[5] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh, Midlothian, Scotland
来源：
VETERINARY JOURNAL | 2014年 / 202卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Porcine epidemic diarrhea virus; Serology; EUSA; S1; protein; Diagnosis; LINKED-IMMUNOSORBENT-ASSAY;
D O I：
10.1016/j.tvjl.2014.07.018
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. In serum samples from experimentally infected pigs (n = 35), anti-IgG PEDV antibodies were detected as early as 7 days post-infection. In field serum samples (n = 239), the diagnostic sensitivity of the S1 ELISA was 100% and the diagnostic specificity was 94%. The Si ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples (n = 133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the Si ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：33 / 36
页数：4
相关论文
共 12 条
[1]   Coronavirus genome structure and replication [J].
Brian, DA ;
Baric, RS .
CORONAVIRUS REPLICATION AND REVERSE GENETICS, 2005, 287 :1-30
[2]   ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR THE DETECTION OF THE CORONAVIRUS-LIKE AGENT AND ITS ANTIBODIES IN PIGS WITH PORCINE EPIDEMIC DIARRHEA [J].
CALLEBAUT, P ;
DEBOUCK, P ;
PENSAERT, M .
VETERINARY MICROBIOLOGY, 1982, 7 (04) :295-306
[3]   EVALUATION OF A BLOCKING ELISA USING MONOCLONAL-ANTIBODIES FOR THE DETECTION OF PORCINE EPIDEMIC DIARRHEA VIRUS AND ITS ANTIBODIES [J].
CARVAJAL, A ;
LANZA, I ;
DIEGO, R ;
RUBIO, P ;
CARMENES, P .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1995, 7 (01) :60-64
[4]  
Ha G.W., 2010, KOREAN J VET PUBLIC, V34, P53
[5]   ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF PORCINE EPIDEMIC DIARRHEA CORONAVIRUS ANTIBODIES IN SWINE SERA [J].
HOFMANN, M ;
WYLER, R .
VETERINARY MICROBIOLOGY, 1990, 21 (03) :263-273
[6]   Development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for detection of porcine epidemic diarrhea (PEDV) antibodies [J].
Hou, Xi-Lin ;
Yu, Li-Yun ;
Liu, Jianzhu .
VETERINARY MICROBIOLOGY, 2007, 123 (1-3) :86-92
[7]   Porcine DC-SIGN: Molecular cloning, gene structure, tissue distribution and binding characteristics [J].
Huang, Y. W. ;
Dryman, B. A. ;
Li, W. ;
Meng, X. J. .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2009, 33 (04) :464-480
[8]   Origin, Evolution, and Genotyping of Emergent Porcine Epidemic Diarrhea Virus Strains in the United States [J].
Huang, Yao-Wei ;
Dickerman, Allan W. ;
Pineyro, Pablo ;
Li, Long ;
Fang, Li ;
Kiehne, Ross ;
Opriessnig, Tanja ;
Meng, Xiang-Jin .
MBIO, 2013, 4 (05)
[9]   AN ELISA FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA VIRUS (PEDV) BASED ON THE SPECIFIC SOLUBILITY OF THE VIRAL SURFACE GLYCOPROTEIN [J].
KNUCHEL, M ;
ACKERMANN, M ;
MULLER, HK ;
KIHM, U .
VETERINARY MICROBIOLOGY, 1992, 32 (02) :117-134
[10]   Vaccination of sows reduces the prevalence of PCV-2 viraemia in their piglets under field conditions [J].
O'Neill, K. C. ;
Hemann, M. ;
Gimenez-Lirola, L. G. ;
Halbur, P. G. ;
Opriessnig, T. .
VETERINARY RECORD, 2012, 171 (17) :425
12