Bleomycin-induced DNA damage and its removal in lymphocytes of breast cancer patients studied by comet assay

被引：90

作者：

Jaloszynski, P

Kujawski, M

Czub-Swierczek, M

Markowska, J

Szyfter, K

机构：

[1] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

[2] Univ Med Sch, Dept Gynecol Oncol, PL-61878 Poznan, Poland

来源：

MUTATION RESEARCH-DNA REPAIR | 1997年 / 385卷 / 03期

关键词：

bleomycin; DNA break; DNA repair; breast cancer; comet assay;

D O I：

10.1016/S0921-8777(97)00046-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The studies concerned the response to bleomycin treatment in peripheral blood lymphocytes (PBL) of breast cancer (BC) subjects. The level of BLM-induced DNA strand breaks was evaluated using alkaline comet assay followed by visual scoring. The sensitivity to genotoxic exposure as well as the time-course of damage removal were estimated and analysed in comparison to control (healthy) subjects. Despite high inter-individual variability, the differences between the BC and non-cancer groups still proved to be statistically significant. Lymphocytes of the BC subjects appeared to be more sensitive to BLM exposure as shown by higher level of DNA damage. The DNA repair capacity was weaker in PBL obtained from BC patients than that in lymphocytes of controls. (C) 1997 Elsevier Science B.V.

引用

页码：223 / 233

页数：11

共 33 条

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