In Vitro Characterization of the Human Skeletal Stem Cell-like Properties of Primary Bone-Derived Mesenchymal Stem/Stromal Cells in Patients with Late and Early Hip Osteoarthritis

被引:5
作者
Jasenc, Lara [1 ]
Strazar, Klemen [2 ,3 ]
Mihelic, Anze [4 ]
Mihalic, Rene [4 ]
Trebse, Rihard [3 ,4 ]
Haring, Gregor [5 ]
Jeras, Matjaz [1 ,6 ]
Zupan, Janja [1 ]
机构
[1] Univ Ljubljana, Fac Pharm, Dept Clin Biochem, Askerceva 7, Ljubljana 1000, Slovenia
[2] Univ Med Ctr Ljubljana, Dept Orthopaed Surg, Zaloska 9, Ljubljana 1000, Slovenia
[3] Univ Ljubljana, Fac Med, Vrazov Trg 2, Ljubljana 1000, Slovenia
[4] Valdoltra Orthopaed Hosp, Jadranska 31, Ankaran 6280, Slovenia
[5] Univ Ljubljana, Fac Med, Inst Forens Med, Korytkova 2, Ljubljana 1000, Slovenia
[6] Biomed Ctr Doo, Celica, Tehnoloski Pk 24, Ljubljana 1000, Slovenia
来源
LIFE-BASEL | 2022年 / 12卷 / 06期
关键词
human skeletal stem cells (hSSCs); late osteoarthritis (OA); early OA; bone-derived mesenchymal stem; stromal cells (MSCs); podoplanin (PDPN); CD73; CD164; CD146; trilineage differentiation; immunophenotyping; IDENTIFICATION; KNEE;
D O I
10.3390/life12060899
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human skeletal stem cells (hSSCs) were recently identified as podoplanin (PDPN)/CD73/CD164-positive and CD146-negative cells that decline with age, and play a role in the pathogenesis of osteoarthritis (OA). The aim of this study was to identify the hSSC-like properties of bone-derived mesenchymal stem/stromal cells (MSCs) of patients with late and early OA. Methods: First, we performed gene expression profiling for the hSSC markers in 32 patients with late and early OA, and donors without OA. Having identified the low expression of hSSC markers in late OA patients, we further performed trilineage differentiation and immunophenotyping for hSSC makers in the selected subsets from each donor group. Results: Our results show no differences in osteogenesis, chondrogenesis, and adipogenesis between the MSCs from the three groups. However, the immunophenotyping shows lower CD164 in MSCs from early OA patients in comparison with late and no OA subjects (p = 0.002 and p = 0.017). Conclusions: Our study shows that the in vitro hSSC-like properties of bone-derived MSCs are similar in patients with early and late OA, and in donors without OA. However, the lower percentage of CD164-positive MSCs in early OA patients indicates the potential of CD164 as a marker of the onset of OA.
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页数:15
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