Cooperativity Between T Cell Receptor Complexes Revealed by Conformational Mutants of CD3ε

被引：73

作者：

Martinez-Martin, Nuria

Risueno, Ruth M.

Morreale, Antonio ^{[1
]}

Zaldivar, Irene

Fernandez-Arenas, Elena

Herranz, Fernando ^{[1
]}

Ortiz, Angel R. ^{[1
]}

Alarcon, Balbino ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Consejo Super Invest Cient, Bioinformat Unit, E-28049 Madrid, Spain

来源：

SCIENCE SIGNALING | 2009年 / 2卷 / 83期

关键词：

PROLINE-RICH SEQUENCE; ANTIGEN RECEPTOR; LENTIVIRAL VECTOR; CRYSTAL-STRUCTURE; ECTODOMAIN; SELECTION; BINDING; MACROMOLECULES; TRANSDUCTION; HETERODIMER;

D O I：

10.1126/scisignal.2000402

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CD3 epsilon subunit of the T cell receptor (TCR) complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. To study this process, we built a molecular dynamics model of the transmission of the conformational change within the ectodomains of CD3. The model showed that the CD3 dimers underwent a stiffening effect that was funneled to the base of the CD3 epsilon subunit. Mutation of two relevant amino acid residues blocked transmission of the conformational change and the differentiation and activation of T cells. Furthermore, this inhibition occurred even in the presence of excess endogenous CD3 epsilon subunits. These results emphasize the importance of the conformational change in CD3 epsilon for the activation of T cells and suggest the existence of unforeseen cooperativity between TCR complexes.

引用

页数：11

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