The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes

被引：39

作者：

Kim, Beom Joon ^{[1
]}

Rho, Yong Kwan ^{[1
]}

Lee, Hye In ^{[1
]}

Jeong, Mi Sook

Li, Kapsok ^{[1
]}

Seo, Seong Jun ^{[1
]}

Kim, Myeung Nam ^{[1
]}

Hong, Chang Kwun ^{[1
]}

机构：

[1] Chung Ang Univ, Dept Dermatol, Coll Med, Chung Ang Med Res Ctr, Seoul 156755, South Korea

来源：

CLINICAL & DEVELOPMENTAL IMMUNOLOGY | 2009年

关键词：

ANTIMICROBIAL PEPTIDES; HUMAN SKIN; 1,25-DIHYDROXYVITAMIN D-3; HUMAN BETA-DEFENSIN-2; CATHELICIDIN LL-37; ATOPIC-DERMATITIS; GENE-EXPRESSION; RETINOIC ACID; IMMUNITY; INDUCTION;

D O I：

10.1155/2009/645898

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Vitamin D has been reported to regulate innate immunity by controlling the expression of antimicrobial peptides (AMPs). Objective. We investigated the effect of calcipotriol on the expression of AMPs in human cultured keratinocytes. Methods. Keratinocytes were treated with lipopolysaccharide (LPS), TNF-alpha, Calcipotriol and irradiated with UVB, cultured, and harvested. To assess the expression of human beta defensin-2 and LL-37 in the control group, not exposed to any stimulants, the experimental group was treated with LPS, TNF-alpha, or UVB, and another group was treated again with calcipotriol; reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemical staining were performed. Results. In the experimental group treated with LPS, UVB irradiation, and TNF-alpha, the expression of beta-defensin and LL-37 was increased more than in the control group and then decreased in the experimental group treated with calcipotriol. Conclusions. Calcipotriol suppressed HBD-2 and LL-37, which were stimulated by UVB, LPS, and TNF-alpha. Copyright (C) 2009 Beom Joon Kim et al.

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页数：8

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