Crystal Structure of the Dog Lipocalin Allergen Can f 2: Implications for Cross-reactivity to the Cat Allergen Fel d 4

被引：60

作者：

Madhurantakam, Chaithanya ^{[1
]}

Nilsson, Ola B. ^{[2
]}

Uchtenhagen, Hannes ^{[1
]}

Konradsen, Jon ^{[3
]}

Saarne, Tiiu ^{[2
]}

Hogbom, Erik ^{[4
]}

Sandalova, Tatyana ^{[5
]}

Gronlund, Hans ^{[6
]}

Achour, Adnane ^{[1
]}

机构：

[1] Karolinska Inst, Karolinska Univ Hosp Huddinge, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden

[2] Karolinska Inst, Dept Med, Clin Immunol & Allergy Unit, Stockholm, Sweden

[3] Karolinska Univ Hosp, Astrid Lindgren Childrens Hosp, Stockholm, Sweden

[4] Phadia AB, Uppsala, Sweden

[5] Karolinska Inst, Dept Med Biophys & Biochem, Stockholm, Sweden

[6] Karolinska Inst, Dept Environm Med, Ctr Allergy Res, Stockholm, Sweden

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2010年 / 401卷 / 01期

关键词：

dog allergen; crystal structure; recombinant Can f 2; epitope; lipocalin fold; RETINOL-BINDING-PROTEIN; BOVINE BETA-LACTOGLOBULIN; 3-DIMENSIONAL STRUCTURE; SALIVARY LIPOCALIN; PHEROMONE BINDING; MAJOR ALLERGEN; TRIGONAL FORM; CRYSTALLIZATION; REFINEMENT; REVEALS;

D O I：

10.1016/j.jmb.2010.05.043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dog lipocalin allergen Can f 2 is an important cause of allergic sensitization in humans worldwide. Here, the first crystal structure of recombinant rCan f 2 at 1.45 angstrom resolution displays a classical lipocalin fold with a conserved Gly-Xaa-Trp motif, in which Trp19 stabilizes the overall topology of the monomeric rCan f 2. Phe38 and Tyr84 localized on the L1 and L5 loops, respectively, control access to the highly hydrophobic calyx. Although the rCan f 2 calyx is nearly identical with the aero-allergens MUP1, Equ c 1 and A2U from mouse, horse and rat, respectively, no IgE cross-reactivity was found using sera from five mono-sensitized subjects. However, clear IgE cross-reactivity was demonstrated between Can f 2 and the cat allergen Fel d 4, although they share less than 22% sequence identity. This suggests a role for these allergens in co-sensitization between cat- and dog-allergic patients. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：68 / 83

页数：16

共 73 条

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