Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction

被引：10

作者：

Guo, Yijie ^{[1
,2
]}

Fukuda, Tomokazu ^{[3
]}

Nakamura, Shuichi ^{[4
]}

Bai, Lanlan ^{[1
]}

Xu, Jun ^{[1
]}

Kuroda, Kengo ^{[1
]}

Tomioka, Rintaro ^{[1
]}

Yoneyama, Hiroshi ^{[1
]}

Isogai, Emiko ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Labs Anim Microbiol, Sendai, Miyagi 9818555, Japan

[2] Xi An Jiao Tong Univ, Sch Med, Dept Immunobiol & Pathogen Biol, Xian 710061, Peoples R China

[3] Tohoku Univ, Lab Anim Breeding & Genet, Sendai, Miyagi 9818555, Japan

[4] Tohoku Univ, Grad Sch Engn, Appl Phys Lab, Sendai, Miyagi 9808579, Japan

来源：

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2015年 / 28卷 / 02期

基金：

日本学术振兴会;

关键词：

Antibody Against Leptospiral Lipopolysaccharide; Cytokines; Leptospiral Lipopolysaccharide; Pig Fibroblast Cell Line; Toll-like Receptor 2; INTERROGANS; EXPRESSION; LPS;

D O I：

10.5713/ajas.14.0440

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

引用

页码：273 / 279

页数：7

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