Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains

被引：99

作者：

Bastien, N

Normand, S

Taylor, T

Ward, D

Peret, TCT

Boivin, G

Anderson, LJ

Li, Y

机构：

[1] Canadian Sci Ctr Human & Anim Hlth, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

[2] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Viral & Rickettsial Dis, Resp & Enter Viruses Branch, Atlanta, GA 30333 USA

[3] Ctr Hosp Univ Quebec, St Foy, PQ G1V 4G2, Canada

[4] Univ Laval, St Foy, PQ G1V 4G2, Canada

来源：

VIRUS RESEARCH | 2003年 / 93卷 / 01期

关键词：

pneumoviruses; hMPV; sequence analysis; phylogenetic relationship;

D O I：

10.1016/S0168-1702(03)00065-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1-97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6-85.7% amino acid identity for isolates between the two clusters, and 94.6-100% for isolates within the same cluster. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：51 / 62

页数：12

共 42 条

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