Simultaneous determination of 16 alkaloids in blood by ultrahigh-performance liquid chromatography-tandem mass spectrometry coupled with supported liquid extraction

被引：19

作者：

Chen, Rui ^{[1
]}

Ning, Ze ^{[2
]}

Zheng, Chao ^{[2
]}

Yang, Yongsheng ^{[1
]}

Zhang, Chengyu ^{[1
]}

Ou, Xuemei ^{[1
]}

Chen, Kehong ^{[1
]}

Yu, Hongyan ^{[1
]}

Wei, Xiaojuan ^{[1
]}

Zhao, Qingbiao ^{[3
]}

He, Jianguo ^{[2
]}

机构：

[1] Alicmgbei Dist Peoples Hosp, Hosp Chongqing Red Cross Soc, Dept Oncol & Hematol, Chongqing 400020, Peoples R China

[2] Jiangbei Dist Peoples Hosp, Hosp Chongqing Red Cross Soc, Chongqing 400020, Peoples R China

[3] East China Normal Univ, Dept Optoelect, Key Lab Polar Mat & Devices MOE, Shanghai 200241, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2019年 / 1128卷

关键词：

Alkaloids; Ultra-high performance liquid chromatography; Tandem mass spectrometry; Supported liquid extraction; HPLC-MS/MS METHOD; SENSITIVE DETERMINATION; HUMAN PLASMA; HUMAN URINE; METABOLITES; QUANTITATION; TROPANE; SAMPLES; MS;

D O I：

10.1016/j.jchromb.2019.121789

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Alkaloids are widely present in food and medicine. However, many alkaloids have poisoning effect. Thus, a sensitive and rapid method for determination of alkaloids is highly desired. In this study, a convenient, reliable and accurate method is developed for the simultaneous determination of 16 poisonous alkaloids in human blood by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with supported liquid extraction (SLE). The 16 alkaloids include colchicine, anisodamine, scopolamine, atropine, sinomenine, dicaine, thebaine, papaverine, strychnine, brucine, aconitine, hypaconitine, yunaconitine, deoxyaconitine, bullatine A and bullatine B. Low limits of detection (LODs) were obtained ranging in 0.002-0.03 mu g L-1. The linear ranges are 0.005-200 mu g L-1 for colchicine, papaverine, brucine, aconitine, hypaconitine, yunaconitine and deoxyaconitine, 0.01-200 mu g L-1 for anisodamine and scopolamine, 0.05-200 mu g L-1 for atropine, strychnine, bullatine A and bullatine B, 0. 1-200 mu g L-1 for other target analytes, all with correlation coefficients (R-2) higher than 0.995. The recoveries range from 79.2% to 95.8%. The relative standard deviation (RSD) values are lower than 15% and the values of matrix effect (ME) range in 86.3%-118.2%. This method of UPLC - MS/MS coupled with SLE has high potential for analyzing multiple alkaloids in blood samples.

引用

页数：6

共 31 条

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