Effect of Supercritical Carbon Dioxide Extraction Parameters on the Biological Activities and Metabolites Present in Extracts from Arthrospira platensis

被引：27

作者：

Esquivel-Hernandez, Diego A. ^{[1
]}

Rodriguez-Rodriguez, Jose ^{[1
]}

Cuellar-Bermudez, Sara P. ^{[2
]}

Saul Garcia-Perez, J. ^{[1
]}

Mancera-Andrade, Elena I. ^{[1
]}

Nunez-Echevarria, Jade E. ^{[3
]}

Ontiveros-Valencia, Aura ^{[1
]}

Rostro-Alanis, Magdalena ^{[1
]}

Garcia-Garcia, Rebeca M. ^{[1
]}

Antonio Torres, J. ^{[1
]}

Chen, Wei Ning ^{[4
]}

Parra-Saldivar, Roberto ^{[1
]}

机构：

[1] Escuela Ingn & Ciencias, Tecnol Monterrey, Campus Monterrey,Ave Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico

[2] KU Leuven Kulak, Lab Aquat Biol, E Sabbelaan 53, B-8500 Kortrijk, Belgium

[3] Tecnol Ambiental Biomex SA CV, Volcan Jorullo 5268, Zapopan, Jalisco 45070, Mexico

[4] Nanyang Technol Univ, Sch Chem & Biomed Engn, 62 Nanyang Dr, Singapore 637457, Singapore

来源：

MARINE DRUGS | 2017年 / 15卷 / 06期

关键词：

Arthrospira platensis; supercritical fluid extraction; antioxidants; antimicrobials; bioactive compounds; nutraceuticals; -linolenic acid; -tocopherol; -carotene; riboflavin; GAMMA-LINOLENIC ACID; MICROWAVE-ASSISTED EXTRACTION; WATER-SOLUBLE VITAMINS; FLUID EXTRACTION; SPIRULINA-PLATENSIS; CO2; EXTRACTION; FATTY-ACIDS; LIQUID-CHROMATOGRAPHY; PHENOLIC CONTENT; ANTIOXIDANT;

D O I：

10.3390/md15060174

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Arthrospira platensis was used to obtain functional extracts through supercritical carbon dioxide extraction (SFE-CO2). Pressure (P), temperature (T), co-solvent (CX), static extraction (SX), dispersant (Di) and dynamic extraction (DX) were evaluated as process parameters through a Plackett-Burman design. The maximum extract yield obtained was 7.48 +/- 0.15% w/w. The maximum contents of bioactive metabolites in extracts were 0.69 +/- 0.09 mu g/g of riboflavin, 5.49 +/- 0.10 mu g/g of -tocopherol, 524.46 +/- 0.10 mu g/g of -carotene, 1.44 +/- 0.10 mu g/g of lutein and 32.11 +/- 0.12 mg/g of fatty acids with 39.38% of palmitic acid, 20.63% of linoleic acid and 30.27% of -linolenic acid. A. platensis extracts had an antioxidant activity of 76.47 +/- 0.71 mu g GAE/g by Folin-Ciocalteu assay, 0.52 +/- 0.02, 0.40 +/- 0.01 and 1.47 +/- 0.02 mu mol TE/g by DPPH, FRAP and TEAC assays, respectively. These extracts showed antimicrobial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Overall, co-solvent was the most significant factor for all measured effects (p < 0.05). Arthrospira platensis represents a sustainable source of bioactive compounds through SFE using the following extraction parameters P: 450 bar, CX: 11 g/min, SX: 15 min, DX: 25 min, T: 60 degrees C and Di: 35 g.

引用

页数：18