Interaction of Ca2+-dependent activator protein for secretion 1 (CAPS1) with septin family proteins in mouse brain

被引：9

作者：

Hosono, Mayu ^{[1
]}

Shinoda, Yo ^{[4
]}

Hirano, Touko ^{[2
]}

Ishizaki, Yasuki ^{[3
]}

Furuichi, Teiichi ^{[4
]}

Sadakata, Tetsushi ^{[1
]}

机构：

[1] Gunma Univ, Grad Sch Med, Adv Sci Res Leaders Dev Unit, 3-39-22 Showa Machi, Maebashi, Gunma 3718511, Japan

[2] Gunma Univ, Grad Sch Med, Educ & Res Support Ctr, Lab Analyt Instruments, Maebashi, Gunma 3718511, Japan

[3] Gunma Univ, Grad Sch Med, Dept Mol & Cellular Neurobiol, Maebashi, Gunma 3718511, Japan

[4] Tokyo Univ Sci, Dept Appl Biol Sci, Noda, Chiba 2788510, Japan

来源：

NEUROSCIENCE LETTERS | 2016年 / 617卷

基金：

日本学术振兴会;

关键词：

Exocytosis; Dense-core vesicle; Protein-protein interaction; Mass spectrometry; Immunoprecipitation; CORE VESICLE TRAFFICKING; NEUROTROPHIN RELEASE; SMALL GTPASES; EXOCYTOSIS; CALCIUM; EXPRESSION; COMPLEX; BINDING; CELLS; MICE;

D O I：

10.1016/j.neulet.2016.02.035

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The Ca2+-dependent activator protein for secretion 1 (CAPS1) protein plays a regulatory role in the dense core vesicle exocytosis pathway. To clarify the functions of this protein in the brain, we searched for novel interaction partners of CAPS1 by mass spectrometry. We identified a specific interaction of CAPS1 with septin family proteins. We also demonstrated that the C-terminal region of the CAPS1 protein binds to part of the deduced GTP-binding domain of septin proteins. It is possible that a tertiary complex of septin, CAPS, and syntaxin contributes to dense-core vesicle trafficking and exocytosis in neurons. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

引用

页码：232 / 235

页数：4

共 31 条

[1] Cyclin-dependent kinase 5 phosphorylation of human septin SEPT5 (hCDCrel-1) modulates exocytosis [J].

Amin, Niranjana D. ;

Zheng, Ya-Li ;

Kesavapany, Sashi ;

Kanungo, Jyotshnabala ;

Guszczynski, Tad ;

Sihag, Ram K. ;

Rudrabhatla, Parvathi ;

Albers, Wayne ;

Grant, Philip ;

Pant, Harish C. .

JOURNAL OF NEUROSCIENCE, 2008, 28 (14) :3631-3643

[2] Novel Ca2+-binding protein (CAPS) related to UNC-31 required for Ca2+-activated exocytosis [J].

Ann, K ;

Kowalchyk, JA ;

Loyet, KM ;

Martin, TFJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (32) :19637-19640

[3] The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex [J].

Beites, CL ;

Campbell, KA ;

Trimble, WS .

BIOCHEMICAL JOURNAL, 2005, 385 :347-353

[4] The septin CDCrel-1 binds syntaxin and inhibits exocytosis [J].

Beites, CL ;

Xie, H ;

Bowser, R ;

Trimble, WS .

NATURE NEUROSCIENCE, 1999, 2 (05) :434-439

[5] CAPS (mammalian UNC-31) protein localizes to membranes involved in dense-core vesicle exocytosis [J].

Berwin, B ;

Floor, E ;

Martin, TFJ .

NEURON, 1998, 21 (01) :137-145

[6] Novel Interactions of CAPS (Ca2+-dependent Activator Protein for Secretion) with the Three Neuronal SNARE Proteins Required for Vesicle Fusion [J].

Daily, Neil J. ;

Boswell, Kristin L. ;

James, Declan J. ;

Martin, Thomas F. J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (46) :35320-35329

[7] Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission [J].

Estey, Mathew P. ;

Di Ciano-Oliveira, Caterina ;

Froese, Carol D. ;

Bejide, Margaret T. ;

Trimble, William S. .

JOURNAL OF CELL BIOLOGY, 2010, 191 (04) :741-749

[8] Ca2+-dependent activator protein for secretion 1 is critical for constitutive and regulated exocytosis but not for loading of transmitters into dense core vesicles [J].

Fujita, Yoshihito ;

Xu, Ainan ;

Xie, Li ;

Arunachalam, Lakshmanan ;

Chou, Ting-Chieh ;

Jiang, Tiandan ;

Chiew, Soon-Kwang ;

Kourtesis, John ;

Wang, Li ;

Gaisano, Herbert Y. ;

Sugita, Shuzo .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (29) :21392-21403

[9] CAPS acts at a prefusion step in dense-core vesicle exocytosis as a PIP2 binding protein [J].

Grishanin, RN ;

Kowalchyk, JA ;

Klenchin, VA ;

Kyougsook, A ;

Earles, CA ;

Chapman, ER ;

Gerona, RRL ;

Martin, TFJ .

NEURON, 2004, 43 (04) :551-562

[10] Expression profiling the human septin gene family [J].

Hall, PA ;

Jung, K ;

Hillan, KJ ;

Russell, SEH .

JOURNAL OF PATHOLOGY, 2005, 206 (03) :269-278

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