RNA-based next generation sequencing in non-small-cell lung cancer in a routine setting: an experience from an Italian referral center

被引：7

作者：

De Luca, Caterina ^{[1
]}

Pepe, Francesco ^{[1
]}

Pisapia, Pasquale ^{[1
]}

Iaccarino, Antonino ^{[1
]}

Righi, Luisella ^{[2
]}

Listi, Angela ^{[2
]}

Russo, Gianluca ^{[1
]}

Campione, Severo ^{[3
]}

Pagni, Fabio ^{[4
]}

Nacchio, Mariantonia ^{[1
]}

Conticelli, Floriana ^{[1
]}

Russo, Maria ^{[1
]}

Fabozzi, Teresa ^{[5
]}

Vigliar, Elena ^{[1
]}

Bellevicine, Claudio ^{[1
]}

Rocco, Danilo ^{[6
]}

Laudati, Stefano ^{[7
]}

Iannaci, Giuseppe ^{[7
]}

Daniele, Bruno ^{[5
]}

Gridelli, Cesare ^{[8
]}

Cortinovis, Diego Luigi ^{[9
]}

Novello, Silvia ^{[2
]}

Molina-Vila, Miguel Angel ^{[10
]}

Rosell, Rafael ^{[10
,11
,12
,13
]}

Troncone, Giancarlo ^{[1
]}

Malapelle, Umberto ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Publ Hlth, Naples, Italy

[2] Univ Turin, San Luigi Hosp, Dept Oncol, Reg Gonzole 1, I-10043 Turin, Italy

[3] Cardarelli Hosp, Dept Adv Technol, Pathol Unit, Naples, Italy

[4] Univ Milan Bicocca UNIMIB, Dept Pathol, Monza, Italy

[5] Osped Mare, Oncol Unit, Naples, Italy

[6] AORN Colli Monaldi, Dept Pulm Oncol, Naples, Italy

[7] ASL Napoli Ctr 1, Unit Pathol, Naples, Italy

[8] SG Moscati Hosp, Div Med Oncol, Avellino, Italy

[9] ASST San Gerardo Hosp Monza, Med Oncol Unit, Monza, Italy

[10] Quiron Dexeus Univ Hosp, Lab Oncol, Pangaea Biotech, Barcelona, Spain

[11] Quiron Dexeus Univ Hosp, Inst Oncol Dr Rosell, Barcelona, Spain

[12] Hosp Badalona Germans Trias & Pujol, Catalan Inst Oncol, Badalona, Spain

[13] Hlth Sci Inst & Hosp, Germans Trias & Pujol, Badalona, Spain

来源：

PERSONALIZED MEDICINE | 2022年 / 19卷 / 05期

关键词：

ALK; IHC; MET; molecular oncology; NGS; NSCLC; NTRK; predictive molecular pathology; RET; ROS1; MUTATIONS; DNA;

D O I：

10.2217/pme-2022-0020

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Aim: ALK, ROS1, NTRK and RET gene fusions and MET exon 14 skipping alterations represent novel predictive biomarkers for advanced non-small-cell lung cancer (NSCLC). Therefore, testing patients for these genetic variants is crucial for choosing the best selective treatment. Over the last couple of decades, next generation sequencing (NGS) platforms have emerged as an extremely useful tool for detecting these variants. Materials & methods: In the present study, we report our NGS molecular records produced during a year of diagnostic activity. Results: Overall, our in-house developed NGS workflow successfully analyzed n = 116/131 (88.5%) NSCLC samples. Of these, eight (6.8%) and five (4.3%) out of 116 patients harbored ALK and RET gene rearrangements, respectively: one case harbored ROS1 gene fusion (0.7%). Conclusion: Our results highlight that an RNA-based NGS analysis can reliably detect gene fusion alterations, thereby playing a pivotal role in the management of NSCLC patients.

引用

页码：395 / 401

页数：7

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