Detection of clothianidin residues in cucumber and apple juice using lateral-flow immunochromatographic assay

被引:9
作者
Li, Hongliang [1 ]
Sun, Baoguo [2 ]
Chen, Tian [1 ]
机构
[1] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian, Shaanxi, Peoples R China
[2] Beijing Technol & Business Univ, Beijing 100083, Peoples R China
基金
中国国家自然科学基金;
关键词
Clothianidin; insecticides; immunochromatographic assay; monoclonal antibody; neonicotinoid; strip test; LINKED-IMMUNOSORBENT-ASSAY; IC-ELISA; NEONICOTINOID INSECTICIDES; MONOCLONAL-ANTIBODY; RAPID DETECTION; STRIP TEST; SOIL; IMMUNOASSAYS; METABOLITES;
D O I
10.1080/09540105.2019.1667309
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Clothianidin (CTA), third generation neonicotinoid insecticide that has been widely used for the long-term control of a wide variety of pests. Therefore, we developed immunochromatographic assay for the detection of CTA residues in cucumber and apple juice. The antigen HCTA-EDC-OVA and the goat anti-mouse IgG antibody were added to a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cutoff limits of the semi-quantitative strip test for CTA were 1 ng/mL in 0.01 M PBS (pH 7.4), 4 ng/mL in cucumber juice, and 5 ng/mL in apple juice, and the results were obtained within 5 min. The immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of CTA residues in cucumber and apple juice.
引用
收藏
页码:1112 / 1122
页数:11
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