Coupling ultra-high-pressure liquid chromatography with mass spectrometry for in-vitro drug-metabolism studies

被引：31

作者：

Spaggiari, Dany ^{[1
]}

Geiser, Laurent ^{[1
]}

Rudaz, Serge ^{[1
]}

机构：

[1] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, CH-1211 Geneva 4, Switzerland

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2014年 / 63卷

关键词：

Drug-drug interaction; In-vitro drug-metabolism; Mass spectrometry (MS); Metabolic stability; Metabolite identification; Metabolite profiling; Reactive metabolite; Sub-2 mu m particle; Ultra-high-pressure liquid chromatography (UHPLC); UHPLC-MS; TIME-OF-FLIGHT; PHARMACEUTICAL ANALYSIS; CAPILLARY APPROACH; SMALL-PARTICLE; UPLC-MS/MS; IDENTIFICATION; CYTOCHROME-P450; THROUGHPUT; MS; QUANTIFICATION;

D O I：

10.1016/j.trac.2014.06.021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review emphasizes the benefits of ultra-high-pressure liquid chromatography (UHPLC) in the context of in-vitro drug-metabolism and pharmacokinetics (DMPK) studies. The analysis throughput afforded by UHPLC is particularly useful during the early DMPK stages, when rapid, reliable data must be generated on a large number of new chemical entities (NCEs) to select the most appropriate drug candidates. UHPLC also offers high chromatographic resolution, an important feature for the identification of unknown metabolites that are potentially generated from NCEs by a few dozen biochemical enzymes and combinations of them. Most DMPK studies require selectivity and peak-assignment certainty that is only afforded by mass spectrometry (MS) detectors. We therefore thoroughly discuss the use of UHPLC-MS and UHPLC-tandem MS with regard to DMPK, highlighting the coupling of UHPLC with quadrupole-based MS analyzers, time-of-flight MS analyzers and hybrid analyzers. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：129 / 139

页数：11

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