ε-Poly-L-lysine production by immobilized cells of Kitasatospora sp MY 5-36 in repeated fed-batch cultures

被引：84

作者：

Zhang, Yang ^{[1
,2
]}

Feng, Xiaohai ^{[1
,2
]}

Xu, Hong ^{[1
,2
]}

Yao, Zhong ^{[1
,2
]}

Ouyang, Pingkai ^{[1
,2
]}

机构：

[1] State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Peoples R China

[2] Nanjing Univ Technol, Coll Food Sci & Light Ind, Nanjing 210009, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 14期

基金：

国家高技术研究发展计划(863计划);

关键词：

Kitasatospora sp MY 5-36; Immobilized cells; Poly-epsilon-lysine; Repeated fed-batch; Loofah sponge; STREPTOMYCES-MARINENSIS; NEOMYCIN PRODUCTION; POLYLYSINE; BIOSYNTHESIS; ACID; MUTANT;

D O I：

10.1016/j.biortech.2010.02.021

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The production of epsilon-PL by Kitasatospora sp. MY 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest epsilon-PL production. Repeated fed-batch cultures for epsilon-PL production were also carried out in a stirred bioreactor and final epsilon-PL concentrations and productivity of 34.11 g L-1 and 9.34 g L-1 d(-1), respectively were achieved by cells immobilize in loofah sponge. These values exceeded those for cultures with free cells (22.53 g L-1 and 3.30 g L-1 d(-1)). The immobilized cells were reused five times over a period of 526 h. These results suggest that the immobilization approach is promising for industrial applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：5523 / 5527

页数：5

共 31 条

[1] Continuous neomycin production by immobilized cells of Streptomyces marinensis NUV-5 in an airlift bioreactor [J].