Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis

被引：28

作者：

Michelon, D. ^{[1
]}

Abraham, S. ^{[1
]}

Ebel, B. ^{[1
]}

De Coninck, J. ^{[1
]}

Husson, F. ^{[1
]}

Feron, G. ^{[2
]}

Gervais, P. ^{[1
]}

Cachon, R. ^{[1
]}

机构：

[1] Univ Bourgogne, AgroSup Dijon, Lab Genie Procedes Microbiol & Alimentaires, F-21065 Dijon, France

[2] FLAVIC ENESAD INRA UB, UMR 1129, Dijon, France

来源：

FEBS JOURNAL | 2010年 / 277卷 / 10期

关键词：

Lactococcus lactis; proton motive force; redox; reducing activity; thiol groups; DISULFIDE OXIDOREDUCTASES; ESCHERICHIA-COLI; PROTEIN THIOLS; REDOX; REDUCTION; OXIDASE; FLPA; CELL; FNR; PH;

D O I：

10.1111/j.1742-4658.2010.07644.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lactococcus lactis can decrease the redox potential at pH 7 (E-h7) from 200 to -200 mV in oxygen free Man-Rogosa-Sharpe media. Neither the consumption of oxidizing compounds or the release of reducing compounds during lactic acid fermentation were involved in the decrease in E-h7 by the bacteria. Thiol groups located on the bacterial cell surface appear to be the main components that are able to establish a greater exchange current between the Pt electrode and the bacteria. After the final E-h7 (-200 mV) was reached, only thiol-reactive reagents could restore the initial E-h7 value. Inhibition of the proton motive force showed no effect on maintaining the final E-h7 value. These results suggest that maintaining the exofacial thiol (-SH) groups in a reduced state does not depend on an active mechanism. Thiol groups appear to be displayed by membrane proteins or cell wall-bound proteins and may participate in protecting cells against oxidative stress.

引用

页码：2282 / 2290

页数：9

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