In situ chondrocyte deformation with physiological compression of the feline patellofemoral joint

被引:50
作者
Clark, AL
Barclay, LD
Matyas, JR
Herzog, W
机构
[1] Univ Calgary, Human Performance Lab, Fac Kinesiol, Calgary, AB T2N 1N4, Canada
[2] Univ Calgary, Fac Med, McCaig Ctr Joint Injury & Arthritis Res, Calgary, AB, Canada
关键词
chondrocyte; aspect ratio; articular cartilage; patellofemoral joint;
D O I
10.1016/S0021-9290(02)00424-4
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The mechanical environment is an important factor affecting the maintenance and adaptation of articular cartilage, and thus the function of the joint and the progression of joint degeneration. Recent evidence suggests that cartilage deformation caused by mechanical loading is directly associated with deformation and volume changes of chondrocytes. Furthermore, in vitro experiments have shown that these changes in the mechanical states of chondrocytes correlate with a change in the biosynthetic activity of cartilage cells. The purpose of this study was to apply our knowledge of contact forces within the feline patellofemoral joint to quantify chondrocyte deformation in situ under loads of physiological magnitude. A uniform, static load of physiological magnitude was applied to healthy articular cartilage still fully intact and attached to its native bone. The compressed cartilage was then chemically fixed to enable the evaluation of cartilage strain, chondrocyte deformation and chondrocyte volumetric fraction. Patella and femoral groove articular cartilages differ in thickness, chondrocyte aspect ratio, and chondrocyte volumetric fraction in both magnitude and depth distribution. Furthermore, when subjected to the same compressive loads, changes to all of these parameters differ in magnitude and depth distribution between patellar and femoral groove articular cartilage. This evidence suggests that significant chondrocyte deformation likely occurs during in vivo joint loading, and may influence chondrocyte biosynthetic activity. Furthermore, we hypothesise that the contrasts between patella and femoral groove cartilages may explain, in part, the site-specific progression of osteoarthritis in the patellofemoral joint of the feline anterior cruciate ligament transected knee. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:553 / 568
页数:16
相关论文
共 37 条
  • [1] ADAMS ME, 1989, J RHEUMATOL, V16, P818
  • [2] Changes in proteoglycan synthesis of chondrocytes in articular cartilage are associated with the time-dependent changes in their mechanical environment
    Bachrach, NM
    Valhmu, WB
    Stazzone, E
    Ratcliffe, A
    Lai, WM
    Mow, VC
    [J]. JOURNAL OF BIOMECHANICS, 1995, 28 (12) : 1561 - 1569
  • [3] BRANDT KD, 1991, ARTHRITIS RHEUM-US, V34, P1560
  • [4] THE EARLY EFFECTS OF JOINT IMMOBILIZATION ON MEDIAL COLLATERAL LIGAMENT HEALING IN AN ACL-DEFICIENT KNEE - A GROSS ANATOMIC AND BIOMECHANICAL INVESTIGATION IN THE ADULT-RABBIT MODEL
    BRAY, RC
    SHRIVE, NG
    FRANK, CB
    CHIMICH, DD
    [J]. JOURNAL OF ORTHOPAEDIC RESEARCH, 1992, 10 (02) : 157 - 166
  • [5] Buschmann MD, 1996, J CELL SCI, V109, P499
  • [6] Contact area and pressure distribution in the feline patellofemoral joint under physiologically meaningful loading conditions
    Clark, AL
    Herzog, W
    Leonard, TR
    [J]. JOURNAL OF BIOMECHANICS, 2002, 35 (01) : 53 - 60
  • [7] CLARK AL, 2001, P 18 C INT SOC BIOM, P162
  • [8] CHONDROMALACIA AND CHRONIC ANTERIOR INSTABILITIES OF THE KNEE
    CONTEDUCA, F
    FERRETTI, A
    MARIANI, PP
    PUDDU, G
    PERUGIA, L
    [J]. AMERICAN JOURNAL OF SPORTS MEDICINE, 1991, 19 (02) : 119 - 123
  • [9] STEREOLOGY FOR ANISOTROPIC CELLS - APPLICATION TO GROWTH CARTILAGE
    CRUZORIVE, LM
    HUNZIKER, EB
    [J]. JOURNAL OF MICROSCOPY, 1986, 143 : 47 - 80
  • [10] FINNEY RL, 1994, CALCULUS, P63