Peroxidase-mediated formation of corn fiber gum-bovine serum albumin conjugates: Molecular and structural characterization

被引：32
作者：
Liu, Yan [1 ]
Yadav, Madhav P. [2 ]
Chau, Hoa K. [2 ]
Qiu, Shuang [2 ]
Zhang, Hui [1 ]
Yin, Lijun [1 ,3 ]
机构：
[1] China Agr Univ, Beijing Key Lab Food Nonthermal Proc, Coll Food Sci & Nutr Engn, POB 40,17 Qinghuadonglu, Beijing 100083, Peoples R China
[2] ARS, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA
[3] Henan Univ Technol, Coll Food Sci & Technol, 140 Songshananlu, Zhengzhou 450052, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2017年 / 166卷
基金：
中国国家自然科学基金;
关键词：
Corn fiber gum; Bovine serum albumin; Intermolecular conjugation; Molecular characterization; Structural characterization; EMULSIFYING PROPERTIES; CROSS-LINKING; MAIZE BRAN; ACACIA GUM; PROTEIN; ARABINOXYLANS; TRANSGLUTAMINASE; POLYSACCHARIDES; CHROMATOGRAPHY; COMPONENTS;
D O I：
10.1016/j.carbpol.2017.02.069
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Corn fiber gum (CFG) was previously conjugated with bovine serum albumin (BSA) via peroxidase treatment to improve its emulsifying properties. The current study was undertaken to evaluate the molecular characteristics of CFG-BSA conjugates prepared at different CFG/BSA weight ratios of 10:1, 4:1,1:1, and 1:4. After the peroxidase treatment, CFG-BSA mixtures at weight ratios of 10:1 and 4:1 showed an increase in molecular weight from 200 and 193 kDa to 218 and 223 kDa respectively. But for CFG-BSA mixtures at weight ratio of 1:4, their molecular weight decreased. Peroxidase treated CFG-BSA mixtures had a more compact but rougher surface morphology as compared with the untreated as revealed by scanning electron microscopy. At lower CFG to BSA weight ratio (1:4), particles of CFG-BSA conjugates appeared to be elongated while at higher CFG to BSA weight ratio (10:1), they were approximately spherical in transmission electron micrographs. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：114 / 122
页数：9
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