Detection of Lymphovascular Invasion in Vulvar Cancer by D2-40 (Podoplanin) as a Predictor for Inguinal Lymph Node Metastases

被引:14
作者
Braun, Michael [1 ,5 ]
Wardelmann, Eva [2 ,5 ]
Debald, Manuel [1 ,5 ]
Walgenbach-Bruenagel, Gisela [3 ,5 ]
Hoeller, Tobias [4 ,5 ]
Wolfgarten, Matthias [1 ,5 ]
Sauerwald, Axel [1 ,5 ]
Rudlowski, Christian [1 ,5 ]
Buettner, Reinhard [2 ,5 ]
Kuhn, Walther [1 ,5 ]
Poelcher, Martin [1 ,5 ]
机构
[1] Univ Bonn, Sch Med, Dept Obstet & Gynecol, D-53105 Bonn, Germany
[2] Univ Bonn, Inst Pathol, D-53105 Bonn, Germany
[3] Univ Bonn, Dept Surg, D-53105 Bonn, Germany
[4] Univ Bonn, Dept Med Stat & Epidemiol, D-53105 Bonn, Germany
[5] Ctr Integrated Oncol CIO Cologne Bonn, Cologne, Germany
来源
ONKOLOGIE | 2009年 / 32卷 / 12期
关键词
Vulvar cancer; Lymphangiosis; D2-40; Regional lymph node metastases; SQUAMOUS-CELL CARCINOMA; BLOOD-VESSEL INVASION; BREAST-CANCER; PROGNOSTIC FACTORS; MONOCLONAL-ANTIBODY; VASCULAR TUMORS; MARKERS; ENDOTHELIUM; IMMUNOHISTOCHEMISTRY; LYMPHANGIOGENESIS;
D O I
10.1159/000252795
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Lymphatic vessel invasion (LVI) plays a major role in the spread of vulvar cancer and predicts regional lymph node metastasis. D2-40, a monoclonal immunohistochemical marker might be able to increase the detection rate of LVI compared to conventional hematoxylin-eosin (HE) staining. The aim of the study was to evaluate the suitability of D2-40 for the prediction of regional lymph node metastases. Patients and Methods: Immunohistochemical staining with D2-40 was performed on formalin-fixed, paraffin-embedded tissue sections of 32 patients with squamous cell carcinoma of the vulva. Slides were screened for the presence of LVI. Correlation with clini-co-pathological features including LVI as retrieved by routine HE-stained sections was assessed. Results: Immunostaining with D2-40 significantly (p = 0.019) increased the frequency of detection of lymphatic invasion compared to conventional HE staining. LVI was correctly identified by D2-40 (D2-40+ LVI) in 65.6% of tumor specimens as compared to 40.6% by routine HE staining (HE+ LVI). D2-40+ LVI significantly (p = 0.026) predicted inguinal lymph node metastases. Conclusions: Immunostaining with D2-40 significantly increased the frequency of detection of lymphatic invasion compared to conventional HE staining in squamous cell carcinomas of the vulva. D2-40+ LVI is a strong predictor for inguinal lymph node metastases.
引用
收藏
页码:732 / 738
页数:7
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