Quantitative investigation of the influence of gold nanoparticles on the dynamics of DNA hybridization using a programmed multi-channel quartz crystal microbalance system

被引：8

作者：

Li, Shaohua ^{[1
]}

Li, Xingchang ^{[1
]}

Zhang, Jianping ^{[1
]}

Zhang, Yakun ^{[1
]}

Han, Jianhua ^{[1
]}

Jiang, Long ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLMS, Key Lab Colloid & Interface Sci, Inst Chem, Beijing 100190, Peoples R China

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2010年 / 364卷 / 1-3期

关键词：

Hybridization; Quartz crystal microbalance; DNA transducer; Gold nanoparticles;

D O I：

10.1016/j.colsurfa.2010.05.016

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This paper describes a quantitative investigation of the influence of gold nanoparticles (AuNPs) on DNA hybridization using a programmed multi-channel quartz crystal microbalance (QCM) device. This system has the benefit of continuous deposition, adsorption, washing, and drying of samples automatically, which saves time and enhances the accuracy of QCM measurements. A demonstration of the hybridization study has been carried out using a DNA fragment of the P53 gene near codon 248. The results demonstrated that the reliability and hybridization sensitivity of the QCM could be significantly improved by introducing AuNPs. The study revealed a significant increase in the apparent hybridization constant (K-D(-1) of 5.29 x 10(10) M-1) with the addition of AuNPs, compared to those obtained without addition of AuNPs (about 10(7) M-1); this effect has not been reported before. The results of the experiment indicate the advantage of the multi-channel QCM chip in studying complicated DNA fixation and hybridization, the conjugating interaction between biomolecules and nanoparticles, and the hybridization dynamics. (C) 2010 Published by Elsevier B.V.

引用

页码：158 / 162

页数：5

共 17 条

[1] Curvature Effects in DNA:Au Nanoparticle Conjugates [J].

Cederquist, Kristin B. ;

Keating, Christine D. .

ACS NANO, 2009, 3 (02) :256-260

[2] Microfluidic chip for fast nucleic acid hybridization [J].

Chung, YC ;

Lin, YC ;

Shiu, MZ ;

Chang, WNT .

LAB ON A CHIP, 2003, 3 (04) :228-233

[3] SELF-ASSEMBLED METAL COLLOID MONOLAYERS - AN APPROACH TO SERS SUBSTRATES [J].

FREEMAN, RG ;

GRABAR, KC ;

ALLISON, KJ ;

BRIGHT, RM ;

DAVIS, JA ;

GUTHRIE, AP ;

HOMMER, MB ;

JACKSON, MA ;

SMITH, PC ;

WALTER, DG ;

NATAN, MJ .

SCIENCE, 1995, 267 (5204) :1629-1632

[4] Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices [J].

Grover, WH ;

Skelley, AM ;

Liu, CN ;

Lagally, ET ;

Mathies, RA .

SENSORS AND ACTUATORS B-CHEMICAL, 2003, 89 (03) :315-323

[5] A three-dimensional heterogeneous DNA sensing surface formed by attaching oligodeoxynucleotide-capped gold nanoparticles onto a gold-coated quartz crystal [J].

Han, SB ;

Lin, JQ ;

Satjapipat, M ;

Baca, AJ ;

Zhou, FM .

CHEMICAL COMMUNICATIONS, 2001, (07) :609-610

[6] Sequential DNA hybridisation assays by fast micromixing [J].

Heule, M ;

Manz, A .

LAB ON A CHIP, 2004, 4 (05) :506-511

[7] The Role Radius of Curvature Plays in Thiolated Oligonucleotide Loading on Gold Nanoparticles [J].

Hill, Haley D. ;

Millstone, Jill E. ;

Banholzer, Matthew J. ;

Mirkin, Chad A. .

ACS NANO, 2009, 3 (02) :418-424

[8] Nanoparticle PCR: Nanogold-assisted PCR with enhanced specificity [J].

Li, HK ;

Huang, JH ;

Lv, JH ;

An, HJ ;

Zhang, XD ;

Zhang, ZZ ;

Fan, CH ;

Hu, J .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (32) :5100-5103

[9] Study on colloidal Au-enhanced DNA sensing by quartz crystal microbalance [J].

Lin, L ;

Zhao, HQ ;

Li, JR ;

Tang, JA ;

Duan, MX ;

Jiang, L .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (03) :817-820

[10] The enhancement effect of gold nanoparticles as a surface modifier on DNA sensor sensitivity [J].

Liu, T ;

Tang, J ;

Jiang, L .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 313 (01) :3-7

← 1 2 →