A Novel Electrochemiluminescence Biosensor Based on Glucose Oxidase Immobized on Magnetic Nanoparticles

被引：31

作者：

Xiong Zhi-Gang ^{[1
]}

Li Jian-Ping ^{[1
]}

Tang Li ^{[2
]}

Cheng Zhi-Qiang ^{[1
]}

机构：

[1] Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, Peoples R China

[2] Guilin Univ Technol, Attached Hosp, Guilin 541004, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 38卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Electrochemiluminescence; Magnetic nanoparticles; Glucose oxidase; Luminol; Chemically modified electrode; FILM; IMMOBILIZATION; SENSOR;

D O I：

10.1016/S1872-2040(09)60053-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel Nano-Fe3O4 particles biosensor for glucose based on luminol electrochemiluminescence (ECL) is presented. Glucose oxidase (GOD) was covalently cross-linked to the surface of synthesized magnetic nano-Fe3O4 particles. Then the composite particles Fe3O4/GOD was adhered to solid paraffin carbon paste electrode by magnetic force to fabricate a working electrode. Hydrogen peroxide was produced by enzymatic reaction of GOD and ECL could be obtained by the reaction between luminol and hydrogen peroxide to construct the renewable glucose biosensor. There was a linear relationship between ECL and glucose concentration in the ranges of 1 x 10(-5) - 1. 0 x 10(-2) mol/L with a regression equation of I = 65. 4374C + 23. 9017 (r = 0. 9987), and the detection limit is 1 mu mol/L. The ECL biosensor has showed short response time and high stability, and the electrode surface was renewable easily. The proposed biosensor has been applied to the determination of glucose in plasma samples.

引用

页码：800 / 804

页数：5

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