F Plasmid TraF and TraH Are Components of an Outer Membrane Complex Involved in Conjugation

被引：28
作者：
Arutyunov, Denis [1 ]
Arenson, Barbara [1 ]
Manchak, Jan [1 ]
Frost, Laura S. [1 ]
机构：
[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
来源：
JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 06期
基金：
加拿大自然科学与工程研究理事会;
关键词：
IV SECRETION SYSTEMS; MATING PAIR STABILIZATION; ESCHERICHIA-COLI; TRANSFER OPERON; TRANSFER REGION; TRANSFER GENE; DNA TRANSFER; SEX FACTOR; PROTEIN; PILUS;
D O I：
10.1128/JB.00726-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
引用
收藏
页码：1730 / 1734
页数：5
相关论文
共 29 条
  • [1] THE ROLE OF THE PILUS IN RECIPIENT CELL RECOGNITION DURING BACTERIAL CONJUGATION MEDIATED BY F-LIKE PLASMIDS
    ANTHONY, KG
    SHERBURNE, C
    SHERBURNE, R
    FROST, LS
    [J]. MOLECULAR MICROBIOLOGY, 1994, 13 (06) : 939 - 953
  • [2] Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: Insights into the mechanism of conjugation
    Anthony, KG
    Klimke, WA
    Manchak, J
    Frost, LS
    [J]. JOURNAL OF BACTERIOLOGY, 1999, 181 (17) : 5149 - 5159
  • [3] The ins and outs of DNA transfer in bacteria
    Chen, I
    Christie, PJ
    Dubnau, D
    [J]. SCIENCE, 2005, 310 (5753) : 1456 - 1460
  • [4] Biogenesis, architecture, and function of bacterial type IV secretion systems
    Christie, PJ
    Atmakuri, K
    Krishnamoorthy, V
    Jakubowski, S
    Cascales, E
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, 2005, 59 : 451 - 485
  • [5] F-pili dynamics by live-cell imaging
    Clarke, Margaret
    Maddera, Lucinda
    Harris, Robin L.
    Silverman, Philip M.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (46) : 17978 - 17981
  • [6] F-like type IV secretion systems encode proteins with thioredoxin folds that are putative DsbC homologues
    Elton, TC
    Holland, SJ
    Frost, LS
    Hazes, B
    [J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (24) : 8267 - 8277
  • [7] Architectures and biogenesis of non-flagellar protein appendages in Gram-negative bacteria
    Fronzes, Remi
    Remaut, Han
    Waksman, Gabriel
    [J]. EMBO JOURNAL, 2008, 27 (17) : 2271 - 2280
  • [8] Structure of a Type IV Secretion System Core Complex
    Fronzes, Remi
    Schafer, Eva
    Wang, Luchun
    Saibil, Helen R.
    Orlova, Elena V.
    Waksman, Gabriel
    [J]. SCIENCE, 2009, 323 (5911) : 266 - 268
  • [9] Mobile genetic elements: The agents of open source evolution
    Frost, LS
    Leplae, R
    Summers, AO
    Toussaint, A
    [J]. NATURE REVIEWS MICROBIOLOGY, 2005, 3 (09) : 722 - 732
  • [10] ANALYSIS OF THE SEQUENCE AND GENE-PRODUCTS OF THE TRANSFER REGION OF THE F-SEX FACTOR
    FROST, LS
    IPPENIHLER, K
    SKURRAY, RA
    [J]. MICROBIOLOGICAL REVIEWS, 1994, 58 (02) : 162 - 210
123