F Plasmid TraF and TraH Are Components of an Outer Membrane Complex Involved in Conjugation

被引：28

作者：

Arutyunov, Denis ^{[1
]}

Arenson, Barbara ^{[1
]}

Manchak, Jan ^{[1
]}

Frost, Laura S. ^{[1
]}

机构：

[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

IV SECRETION SYSTEMS; MATING PAIR STABILIZATION; ESCHERICHIA-COLI; TRANSFER OPERON; TRANSFER REGION; TRANSFER GENE; DNA TRANSFER; SEX FACTOR; PROTEIN; PILUS;

D O I：

10.1128/JB.00726-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.

引用

页码：1730 / 1734

页数：5

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