Detection of antibodies against the classical swine fever virus by enzyme-linked immunosorbent assay (ELISA)

被引:0
作者
Islas, A
Quinteros, G
Flores, M
Quezada, M
Diaz, N
Sierra, M
机构
[1] Univ Concepcion, Fac Med Vet, Dept Ciencias Clin, Chillan, Chile
[2] Univ Concepcion, Fac Med Vet, Dept Patol Anim, Chillan, Chile
[3] Serv Agricola & Ganadero, Dept Labs, Santiago, Chile
[4] Univ Cordoba, Fac Vet, Dept Anat & Anat Patol Comparadas, E-14071 Cordoba, Spain
关键词
classical swine fever; diagnosis; ELISA;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Classical swine fever (CSF) is an important worldwide disease. In Chile, it is subject to a control and erradication programme. The objective of the present assay was to standardize an immunoenzimatic technique (CIV-ELISA) that detects antibodies of the IgG type directed against the CSF virus and to compare it with seroneutralization. Twenty hybrid Landrace x Large White pigs with a live weight of 20 kg were used. They were experimentally inoculated with a dose of 100.000 TCID(50) of the Quillota virulent isolate of the CSF virus. A four-pig group was vaccinated 14 days before inoculation. Blood samples were taken 3, 6, 9, 12 and 14 days post-inoculation. Serological samples were obtained from 100 sows. The anti-CSF antibodies were determined for a policlonal CIV-ELISA and were compared with a seroneutralization test (serological official test). The CIV-ELISA test did not detect antibodies in the experimentally inoculated pigs until day 14 post-inoculation. However, the positive-sera condition of previously vaccinated pigs was ratified by the seroneutralization test. The sensibility and specificity of the CIV-ELISA was about 90.1% and 76.4% respectively. The CIV-ELISA is an easy-to-apply test and the results obtained are similar to those obtained with seroneutralization.
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页码:213 / 220
页数:8
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