Singe cell genomes of Prochlorococcus, Synechococcus, and sympatric microbes from diverse marine environments

被引：63

作者：

Berube, Paul M. ^{[1
]}

Biller, Steven J. ^{[1
,13
]}

Hackl, Thomas ^{[1
]}

Hogle, Shane L. ^{[1
]}

Satinsky, Brandon M. ^{[1
]}

Becker, Jamie W. ^{[1
]}

Braakman, Rogier ^{[1
]}

Collins, Sara B. ^{[1
]}

Kelly, Libusha ^{[2
]}

Berta-Thompson, Jessie ^{[1
]}

Coe, Allison ^{[1
]}

Bergauer, Kristin ^{[3
]}

Bouman, Heather A. ^{[4
]}

Browning, Thomas J. ^{[5
]}

De Corte, Daniele ^{[6
]}

Hassler, Christel ^{[7
]}

Hulata, Yotam ^{[8
]}

Jacquot, Jeremy E. ^{[9
]}

Maas, Elizabeth W. ^{[10
]}

Reinthaler, Thomas ^{[3
]}

Sintes, Eva ^{[3
]}

Yokokawa, Taichi ^{[6
]}

Lindell, Debbie ^{[8
]}

Stepanauskas, Ramunas ^{[11
]}

Chisholm, Sallie W. ^{[1
,12
]}

机构：

[1] MIT, Dept Civil & Environm Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[2] Albert Einstein Coll Med, Dept Syst & Computat Biol, Dept Microbiol & Immunol, Bronx, NY 10461 USA

[3] Univ Vienna, Dept Limnol & Biooceanog, A-1090 Vienna, Austria

[4] Univ Oxford, Dept Earth Sci, Oxford OX1 3AN, England

[5] GEOMAR Helmholtz Ctr Ocean Res, Marine Biogeochem Div, D-24148 Kiel, Germany

[6] Japan Agcy Marine Earth Sci & Technol, Res & Dev Ctr Marine Biosci, 2-15 Natsushimacho, Yokosuka, Kanagawa 2370061, Japan

[7] Univ Geneva, Dept FA Forel Environm & Aquat Sci, CH-1211 Geneva, Switzerland

[8] Technion Israel Inst Technol, Fac Biol, IL-3200003 Haifa, Israel

[9] Univ Southern Calif, Dept Biol Sci, Los Angeles, CA 90089 USA

[10] Minist Primary Ind, Napier 4144, New Zealand

[11] Bigelow Lab Ocean Sci, Single Cell Genom Ctr, East Boothbay, ME 04544 USA

[12] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[13] Wellesley Coll, Dept Biol Sci, Wellesley, MA 02481 USA

来源：

SCIENTIFIC DATA | 2018年 / 5卷

基金：

美国国家科学基金会;

关键词：

ALGORITHM; SEQUENCE; OCEAN;

D O I：

10.1038/sdata.2018.154

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Prochlorococcus and Synechococcus are the dominant primary producers in marine ecosystems and perform a significant fraction of ocean carbon fixation. These cyanobacteria interact with a diverse microbial community that coexists with them. Comparative genomics of cultivated isolates has helped address questions regarding patterns of evolution and diversity among microbes, but the fraction that can be cultivated is miniscule compared to the diversity in the wild. To further probe the diversity of these groups and extend the utility of reference sequence databases, we report a data set of single cell genomes for 489 Prochlorococcus, 50 Synechococcus, 9 extracellular virus particles, and 190 additional microorganisms from a diverse range of bacterial, archaeal, and viral groups. Many of these uncultivated single cell genomes are derived from samples obtained on GEOTRACES cruises and at well-studied oceanographic stations, each with extensive suites of physical, chemical, and biological measurements. The genomic data reported here greatly increases the number of available Prochlorococcus genomes and will facilitate studies on evolutionary biology, microbial ecology, and biological oceanography.

引用

页数：11

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