Engineering and monitoring cellular barrier models

被引:59
作者
Yeste, Jose [1 ,2 ]
Illa, Xavi [1 ,2 ]
Alvarez, Mar [1 ]
Villa, Rosa [1 ,2 ]
机构
[1] CSIC, CNM, IMB, Barcelona 08193, Spain
[2] CIBER BBN, Barcelona, Spain
关键词
Biological barriers; Cell barrier function; Microphysiological systems; Transepithelial electrical properties; BLOOD-BRAIN-BARRIER; ON-A-CHIP; IN-VITRO MODEL; EPITHELIAL ELECTRICAL-RESISTANCE; IMPEDANCE SENSING ECIS; HUMAN INTESTINAL-CELLS; EXTRACELLULAR-MATRIX; TIGHT JUNCTIONS; SHEAR-STRESS; MICROFLUIDIC MODEL;
D O I
10.1186/s13036-018-0108-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Epithelia and endothelia delineate tissue compartments and control their environments by regulating the passage of ions and solutes. This barrier function is essential for the development and maintenance of multicellular organisms, and its dysfunction is associated with numerous human diseases. Recent advances in biomaterials and microfabrication technologies have evolved in vitro approaches for modelling biological barriers. Current microphysiological systems have become more efficient and reliable in mimicking the cell microenvironment. Additionally, methods for the quantification of barrier permeability have long provided significant insight into their underlying mechanisms. In this review, we outline the current techniques to quantify the barrier function of engineered tissues, and we also give an overview of recent microphysiological systems of biological barriers that emulate the microenvironment and microarchitecture of native tissues.
引用
收藏
页数:19
相关论文
共 135 条
[41]   Tight junctions and the modulation of barrier function in disease [J].
Foerster, Carola .
HISTOCHEMISTRY AND CELL BIOLOGY, 2008, 130 (01) :55-70
[42]   Differential effects of hydrocortisone and TNFα on tight junction proteins in an in vitro model of the human blood-brain barrier [J].
Foerster, Carola ;
Burek, Malgorzata ;
Romero, Ignacio A. ;
Weksler, Babette ;
Couraud, Pierre-Olivier ;
Drenckbahni, Detlev .
JOURNAL OF PHYSIOLOGY-LONDON, 2008, 586 (07) :1937-1949
[43]   Real-time quantitative monitoring of hiPSC-based model of macular degeneration on Electric Cell-substrate Impedance Sensing microelectrodes [J].
Gamal, W. ;
Borooah, S. ;
Smith, S. ;
Underwood, I. ;
Srsen, V. ;
Chandran, S. ;
Bagnaninchi, P. O. ;
Dhillon, B. .
BIOSENSORS & BIOELECTRONICS, 2015, 71 :445-455
[44]   Extracellular matrix: A dynamic microenvironment for stem cell niche [J].
Gattazzo, Francesca ;
Urciuolo, Anna ;
Bonaldo, Paolo .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2014, 1840 (08) :2506-2519
[45]   Environmental sensing through focal adhesions [J].
Geiger, Benjamin ;
Spatz, Joachim P. ;
Bershadsky, Alexander D. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (01) :21-33
[46]   USE OF ELECTRIC-FIELDS TO MONITOR THE DYNAMIC ASPECT OF CELL BEHAVIOR IN TISSUE-CULTURE [J].
GIAEVER, I ;
KEESE, CR .
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 1986, 33 (02) :242-247
[47]   Hepatic sinusoids in liver injury, inflammation, and fibrosis: new pathophysiological insights [J].
Greuter, Thomas ;
Shah, Vijay H. .
JOURNAL OF GASTROENTEROLOGY, 2016, 51 (06) :511-519
[48]   MORPHOGENETIC INTERACTION BETWEEN EMBRYONIC MOUSE TISSUES SEPARATED BY A MEMBRANE FILTER [J].
GROBSTEIN, C .
NATURE, 1953, 172 (4384) :869-871
[49]  
Hamalainen KM, 1997, J CONTROL RELEASE, V49, P97
[50]   Development of a three-dimensional, all-human in vitro model of the blood-brain barrier using mono-, co-, and tri-cultivation Transwell models [J].
Hatherell, Kathryn ;
Couraud, Pierre-Olivier ;
Romero, Ignacio A. ;
Weksler, Babette ;
Pilkington, Geoffrey J. .
JOURNAL OF NEUROSCIENCE METHODS, 2011, 199 (02) :223-229