New strategy for the synthesis of 3′,5′-bifunctionalized oligonucleotide conjugates through sequential formation of chemoselective oxime bonds

被引：46

作者：

Edupuganti, OP ^{[1
]}

Singh, Y ^{[1
]}

Defrancq, E ^{[1
]}

Dumy, P ^{[1
]}

机构：

[1] Univ Grenoble 1, LEDSS, CNRS, UMR5616,ICMG FR2607, F-38041 Grenoble 9, France

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2004年 / 10卷 / 23期

关键词：

chemoselectivity; conjugation; DNA; oligonucleotides; oxime; peptides;

D O I：

10.1002/chem.200400390

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A convenient strategy for the synthesis of bifunctionalized oligonucleotide conjugates bearing two different reporters at the 3' and 5' ends of the oligonucleotide is presented. The method involves the preparation of oligonucleotides bearing an aldehyde and/or aminooxy functionality at each end, followed by reaction to form oxime bonds with appropriately functionalized reporters. The conjugation reactions are carried out under mild aqueous conditions with good reaction yield.

引用

页码：5988 / 5995

页数：8

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