Green fluorescent protein as a reporter for the spatial and temporal expression of actIII in Streptomyces coelicolor

被引:3
|
作者
Santos-Beneit, Fernando [1 ]
Errington, Jeff [1 ]
机构
[1] Newcastle Univ, Ctr Bacterial Cell Biol, Inst Cell & Mol Biosci, Med Sch, Newcastle Upon Tyne, Tyne & Wear, England
基金
英国惠康基金;
关键词
Polyketides; Actinorhodin; Streptomyces coelicolor; ActIII; ActA; SCO5086; SCO5083; AROMATIC POLYKETIDE SYNTHASE; ACTINORHODIN BIOSYNTHESIS; GENE-CLUSTER; A3(2); TRANSCRIPTION; EXPORT; STEPS;
D O I
10.1007/s00203-017-1358-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polyketides constitute a large group of structurally diverse natural products with useful biological activities. Insights into their biosynthetic mechanisms are crucial for developing new structures. One of the most studied model polyketide is the blue-pigmented antibiotic actinorhodin, produced by Streptomyces coelicolor. This aromatic polyketide is synthesized by minimal type II polyketide synthases and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis. Previous papers have reported contradictory findings for localization of the protein in the cytoplasmic fraction or associated with the cell wall. We have now used green fluorescent protein as a reporter to analyse the spatial and temporal expression of actIII (SCO5086) in S. coelicolor under actinorhodin producing and non-producing conditions. We provide evidence in support of ActIII being a cytosolic protein, with limited if any association with the membrane or cell wall.
引用
收藏
页码:875 / 880
页数:6
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