Association between probiotics and enteral nutrition in an experimental acute pancreatitis model in rats

被引：30

作者：

van Baal, Mark C. ^{[1
]}

van Rens, Michiel J. ^{[1
]}

Geven, Christopher B. ^{[1
]}

van de Pol, Francien M. ^{[2
]}

van den Brink, Ilona W. ^{[2
]}

Hannink, Gerjon ^{[1
]}

Nagtegaal, Iris D. ^{[3
]}

Peters, Wilbert H. ^{[4
]}

Rijkers, Ger T. ^{[1
]}

Gooszen, Hein G. ^{[1
]}

机构：

[1] Radboudumc, Dept Operating Rooms Evidence Based Surg, NL-6500 MB Nijmegen, Netherlands

[2] Radboudumc, Dept Anesthesiol, NL-6500 MB Nijmegen, Netherlands

[3] Radboudumc, Dept Pathol, NL-6500 MB Nijmegen, Netherlands

[4] Radboudumc, Dept Gastroenterol, NL-6500 MB Nijmegen, Netherlands

来源：

PANCREATOLOGY | 2014年 / 14卷 / 06期

关键词：

Acute pancreatitis; Probiotics; Prophylaxis; Enteral nutrition; Bowel ischaemia; Bacterial translocation; INTESTINAL BARRIER DYSFUNCTION; NECROTIZING PANCREATITIS; GLUTATHIONE BIOSYNTHESIS; BACTERIAL TRANSLOCATION; ORGAN FAILURE; NECROSECTOMY; INFECTION;

D O I：

10.1016/j.pan.2014.10.002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/objectives: Recently, a randomized controlled trial showed that probiotic prophylaxis was associated with an increased mortality in enterally fed patients with predicted severe pancreatitis. In a rat model for acute pancreatitis, we investigated whether an association between probiotic prophylaxis and enteral nutrition contributed to the higher mortality rate. Methods: Male Sprague-Dawley rats were allocated to four groups: 1) acute pancreatitis (n = 9), 2) acute pancreatitis and probiotic prophylaxis (n = 10), 3) acute pancreatitis and enteral nutrition (n = 10), and 4) acute pancreatitis, probiotic prophylaxis and enteral nutrition (n = 11). Acute pancreatitis was induced by intraductal glycodeoxycholate and intravenous cerulein infusion. Enteral nutrition, saline, probiotics and placebo were administered through a permanent jejunal feeding. Probiotics or placebo were administered starting 4 days before induction of pancreatitis and enteral nutrition 1 day before start until the end of the experiment, 6 days after induction of pancreatitis. Tissue samples and body fluids were collected for microbiological and histological examination. Results: In all animals, serum amylase was increased six hours after induction of pancreatitis. After fulfilling the experiment, no differences between groups were found in histological severity of pancreatitis, degree of discomfort, weight loss, histological examination of small bowel and bacterial translocation (all p > 0.05). Overall mortality was 10% without differences between groups (p = 0.54). Conclusion: No negative association was found between prophylactic probiotics and enteral nutrition in acute pancreatitis. No new clues for a potential mechanism responsible for the higher mortality and bowel ischaemia in the PROPATRIA study were found. Copyright (C) 2014, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved.

引用

页码：470 / 477

页数：8

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