A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway

被引:15
作者
Bories, Pierre [1 ]
Rikardsen, Audun H. [2 ]
Leonards, Pim [3 ]
Fisk, Aaron T. [4 ]
Tartu, Sabrina [5 ]
Vogel, Emma F. [2 ]
Bytingsvik, Jenny [1 ]
Blevin, Pierre [1 ]
机构
[1] Akvaplan Niva AS, Fram Ctr, NO-9296 Tromso, Norway
[2] UiT Arctic Univ Norway, Dept Arctic & Marine Biol, Tromso, Norway
[3] Vrije Univ, Dept Environm & Hlth, Amsterdam, Netherlands
[4] Univ Windsor, Sch Environm, Windsor, ON, Canada
[5] Ctr Etud Biol Chize, Villiers En Bois, France
关键词
capital breeder; cetacean; herring eater; income breeder; LION; web; lipid; seal eater; stable isotope; STABLE-ISOTOPES; SEASONAL PATTERNS; ORCINUS-ORCA; DIET; MIGRATION; TISSUE; NICHE; CONTAMINANTS; POPULATIONS; DIVERGENCE;
D O I
10.1002/ece3.7523
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
In cetaceans, blubber is the primary and largest lipid body reservoir. Our current understanding about lipid stores and uses in cetaceans is still limited, and most studies only focused on a single narrow snapshot of the lipidome. We documented an extended lipidomic fingerprint in two cetacean species present in northern Norway during wintertime. We were able to detect 817 molecular lipid species in blubber of killer whales (Orcinus orca) and humpback whales (Megaptera novaeangliae). The profiles were largely dominated by triradylglycerols in both species and, to a lesser extent, by other constituents including glycerophosphocholines, phosphosphingolipids, glycerophosphoethanolamines, and diradylglycerols. Through a unique combination of traditional statistical approaches, together with a novel bioinformatic tool (LION/web), we showed contrasting fingerprint composition between species. The higher content of triradylglycerols in humpback whales is necessary to fuel their upcoming half a year fasting and energy-demanding migration between feeding and breeding grounds. In adipocytes, we assume that the intense feeding rate of humpback whales prior to migration translates into an important accumulation of triacylglycerol content in lipid droplets. Upstream, the endoplasmic reticulum is operating at full capacity to supply acute lipid storage, consistent with the reported enrichment of glycerophosphocholines in humpback whales, major components of the endoplasmic reticulum. There was also an enrichment of membrane components, which translates into higher sphingolipid content in the lipidome of killer whales, potentially as a structural adaptation for their higher hydrodynamic performance. Finally, the presence of both lipid-enriched and lipid-depleted individuals within the killer whale population in Norway suggests dietary specialization, consistent with significant differences in delta N-15 and delta C-13 isotopic ratios in skin between the two groups, with higher values and a wider niche for the lipid-enriched individuals. Results suggest the lipid-depleted killer whales were herring specialists, while the lipid-enriched individuals might feed on both herrings and seals.
引用
收藏
页码:6716 / 6729
页数:14
相关论文
共 89 条
[81]   MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis [J].
Tsugawa, Hiroshi ;
Cajka, Tomas ;
Kind, Tobias ;
Ma, Yan ;
Higgins, Brendan ;
Ikeda, Kazutaka ;
Kanazawa, Mitsuhiro ;
VanderGheynst, Jean ;
Fiehn, Oliver ;
Arita, Masanori .
NATURE METHODS, 2015, 12 (06) :523-+
[82]   Membrane curvature sorts lipids - Stabilized lipid rafts in membrane transport [J].
van Meer, G .
EMBO REPORTS, 2005, 6 (05) :418-419
[83]   Cellular lipidomics [J].
van Meer, G .
EMBO JOURNAL, 2005, 24 (18) :3159-3165
[84]   Membrane lipids: where they are and how they behave [J].
van Meer, Gerrit ;
Voelker, Dennis R. ;
Feigenson, Gerald W. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (02) :112-124
[85]   Lipid map of the mammalian cell [J].
van Meer, Gerrit ;
de Kroon, Anton I. P. M. .
JOURNAL OF CELL SCIENCE, 2011, 124 (01) :5-8
[86]   Prey switching by killer whales in the north-east Atlantic: observational evidence and experimental insights [J].
Vongraven, Dag ;
Bisther, Anna .
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM, 2014, 94 (06) :1357-1365
[88]   Vertical distribution of lipids, fatty acids and organochlorine contaminants in the blubber of southern hemisphere humpback whales (Megaptera novaeangliae) [J].
Waugh, Courtney A. ;
Nichols, Peter D. ;
Schlabach, Martin ;
Noad, Michael ;
Nash, Susan Bengtson .
MARINE ENVIRONMENTAL RESEARCH, 2014, 94 :24-31
[89]   Lipid and fatty acid profiles of migrating Southern Hemisphere humpback whales Megaptera novaeangliae [J].
Waugh, Courtney A. ;
Nichols, Peter D. ;
Noad, Michael C. ;
Nash, Susan Bengtson .
MARINE ECOLOGY PROGRESS SERIES, 2012, 471 :271-281