Direct dating of Neanderthal remains from the site of Vindija Cave and implications for the Middle to Upper Paleolithic transition

被引:83
作者
Deviese, Thibaut [1 ]
Karavanic, Ivor [2 ,3 ]
Comeskey, Daniel [1 ]
Kubiak, Cara [1 ]
Korlevic, Petra [4 ]
Hajdinjak, Mateja [4 ]
Radovic, Sinisa [5 ]
Procopio, Noemi [6 ]
Buckley, Michael [6 ]
Paabo, Svante [4 ]
Higham, Tom [1 ]
机构
[1] Univ Oxford, Oxford Radiocarbon Accelerator Unit, Res Lab Archaeol & Hist Art, Oxford OX1 3QY, England
[2] Univ Zagreb, Dept Archaeol, Fac Humanities & Social Sci, HR-10000 Zagreb, Croatia
[3] Univ Wyoming, Dept Anthropol, Laramie, WY 82071 USA
[4] Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany
[5] Croatian Acad Sci & Arts, Inst Quaternary Palaeontol & Geol, HR-10000 Zagreb, Croatia
[6] Univ Manchester, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
基金
欧洲研究理事会;
关键词
Vindija Cave (Croatia); single-compound AMS dating; DNA analysis; zooarchaeology by mass spectrometry; Middle to Upper Paleolithic transition; MITOCHONDRIAL GENOME SEQUENCE; SPECIES IDENTIFICATION; RADIOCARBON-DATES; VELIKA PECINA; MODERN HUMANS; DNA; ANCIENT; INTERFACE; CROATIA; EUROPE;
D O I
10.1073/pnas.1709235114
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Previous dating of the Vi-207 and Vi-208 Neanderthal remains from Vindija Cave (Croatia) led to the suggestion that Neanderthals survived there as recently as 28,000-29,000 B.P. Subsequent dating yielded older dates, interpreted as ages of at least similar to 32,500 B.P. We have redated these same specimens using an approach based on the extraction of the amino acid hydroxyproline, using preparative high-performance liquid chromatography (Prep-HPLC). This method is more efficient in eliminating modern contamination in the bone collagen. The revised dates are older than 40,000 B.P., suggesting the Vindija Neanderthals did not live more recently than others across Europe, and probably predate the arrival of anatomically modern humans in Eastern Europe. We applied zooarchaeology by mass spectrometry (ZooMS) to find additional hominin remains. We identified one bone that is Neanderthal, based on its mitochondrial DNA, and dated it directly to 46,200 +/- 1,500 B.P. We also attempted to date six early Upper Paleolithic bone points from stratigraphic units G(1), Fd/d+G(1) and Fd/d, Fd. One bone artifact gave a date of 29,500 +/- 400 B.P., while the remainder yielded no collagen. We additionally dated animal bone samples from units G1 and G(1)-G(3). These dates suggest a co-occurrence of early Upper Paleolithic osseous artifacts, particularly split-based points, alongside the remains of Neanderthals is a result of postdepositional mixing, rather than an association between the two groups, although more work is required to show this definitively.
引用
收藏
页码:10606 / 10611
页数:6
相关论文
共 52 条
  • [11] An early modern human from Romania with a recent Neanderthal ancestor
    Fu, Qiaomei
    Hajdinjak, Mateja
    Moldovan, Oana Teodora
    Constantin, Silviu
    Mallick, Swapan
    Skoglund, Pontus
    Patterson, Nick
    Rohland, Nadin
    Lazaridis, Iosif
    Nickel, Birgit
    Viola, Bence
    Pruefer, Kay
    Meyer, Matthias
    Kelso, Janet
    Reich, David
    Paeaebo, Svante
    [J]. NATURE, 2015, 524 (7564) : 216 - +
  • [12] DNA analysis of an early modern human from Tianyuan Cave, China
    Fu, Qiaomei
    Meyer, Matthias
    Gao, Xing
    Stenzel, Udo
    Burbano, Hernan A.
    Kelso, Janet
    Paeaebo, Svante
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (06) : 2223 - 2227
  • [13] Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase
    Gansauge, Marie-Theres
    Gerber, Tobias
    Glocke, Isabelle
    Korlevic, Petra
    Lippik, Laurin
    Nagel, Sarah
    Riehl, Lara Maria
    Schmidt, Anna
    Meyer, Matthias
    [J]. NUCLEIC ACIDS RESEARCH, 2017, 45 (10)
  • [14] Selective enrichment of damaged DNA molecules for ancient genome sequencing
    Gansauge, Marie-Theres
    Meyer, Matthias
    [J]. GENOME RESEARCH, 2014, 24 (09) : 1543 - 1549
  • [15] Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
    Gansauge, Marie-Theres
    Meyer, Matthias
    [J]. NATURE PROTOCOLS, 2013, 8 (04) : 737 - 748
  • [16] A complete neandertal mitochondrial genome sequence determined by high-throughput Sequencing
    Green, Richard E.
    Malaspinas, Anna-Sapfo
    Krause, Johannes
    Briggs, Adrian W.
    Johnson, Philip L. F.
    Uhler, Caroline
    Meyer, Matthias
    Good, Jeffrey M.
    Maricic, Tomislav
    Stenzel, Udo
    Pruefer, Kay
    Siebauer, Michael
    Burbano, Hernan A.
    Ronan, Michael
    Rothberg, Jonathan M.
    Egholm, Michael
    Rudan, Pavao
    Brajkovic, Dejana
    Kucan, Zeljko
    Gusic, Ivan
    Wikstrom, Marten
    Laakkonen, Liisa
    Kelso, Janet
    Slatkin, Montgomery
    Paeaebo, Svante
    [J]. CELL, 2008, 134 (03) : 416 - 426
  • [17] A Draft Sequence of the Neandertal Genome
    Green, Richard E.
    Krause, Johannes
    Briggs, Adrian W.
    Maricic, Tomislav
    Stenzel, Udo
    Kircher, Martin
    Patterson, Nick
    Li, Heng
    Zhai, Weiwei
    Fritz, Markus Hsi-Yang
    Hansen, Nancy F.
    Durand, Eric Y.
    Malaspinas, Anna-Sapfo
    Jensen, Jeffrey D.
    Marques-Bonet, Tomas
    Alkan, Can
    Pruefer, Kay
    Meyer, Matthias
    Burbano, Hernan A.
    Good, Jeffrey M.
    Schultz, Rigo
    Aximu-Petri, Ayinuer
    Butthof, Anne
    Hoeber, Barbara
    Hoeffner, Barbara
    Siegemund, Madlen
    Weihmann, Antje
    Nusbaum, Chad
    Lander, Eric S.
    Russ, Carsten
    Novod, Nathaniel
    Affourtit, Jason
    Egholm, Michael
    Verna, Christine
    Rudan, Pavao
    Brajkovic, Dejana
    Kucan, Zeljko
    Gusic, Ivan
    Doronichev, Vladimir B.
    Golovanova, Liubov V.
    Lalueza-Fox, Carles
    de la Rasilla, Marco
    Fortea, Javier
    Rosas, Antonio
    Schmitz, Ralf W.
    Johnson, Philip L. F.
    Eichler, Evan E.
    Falush, Daniel
    Birney, Ewan
    Mullikin, James C.
    [J]. SCIENCE, 2010, 328 (5979) : 710 - 722
  • [18] Revised direct radiocarbon dating of the Vindija G1 upper Paleolithic Neandertals
    Higham, T
    Ramsey, CB
    Karavanic, I
    Smith, FH
    Trinkaus, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (03) : 553 - 557
  • [19] European Middle and Upper Palaeolithic radiocarbon dates are often older than they look: problems with previous dates and some remedies
    Higham, Thomas
    [J]. ANTIQUITY, 2011, 85 (327) : 235 - 249
  • [20] The timing and spatiotemporal patterning of Neanderthal disappearance
    Higham, Tom
    Douka, Katerina
    Wood, Rachel
    Ramsey, Christopher Bronk
    Brock, Fiona
    Basell, Laura
    Camps, Marta
    Arrizabalaga, Alvaro
    Baena, Javier
    Barroso-Ruiz, Cecillio
    Bergman, Christopher
    Boitard, Coralie
    Boscato, Paolo
    Caparros, Miguel
    Conard, Nicholas J.
    Draily, Christelle
    Froment, Alain
    Galvan, Bertila
    Gambassini, Paolo
    Garcia-Moreno, Alejandro
    Grimaldi, Stefano
    Haesaerts, Paul
    Holt, Brigitte
    Iriarte-Chiapusso, Maria-Jose
    Jelinek, Arthur
    Jorda Pardo, Jesus F.
    Maillo-Fernandez, Jose-Manuel
    Marom, Anat
    Maroto, Julia
    Menendez, Mario
    Metz, Laure
    Morin, Eugene
    Moroni, Adriana
    Negrino, Fabio
    Panagopoulou, Eleni
    Peresani, Marco
    Pirson, Stephane
    de la Rasilla, Marco
    Riel-Salvatore, Julien
    Ronchitelli, Annamaria
    Santamaria, David
    Semal, Patrick
    Slimak, Ludovic
    Soler, Joaquim
    Soler, Narcis
    Villaluenga, Aritza
    Pinhasi, Ron
    Jacobi, Roger
    [J]. NATURE, 2014, 512 (7514) : 306 - 309