Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration

被引：20

作者：

Yin, Bo ^{[1
,2
]}

Ma, Pei ^{[1
,2
,3
,4
]}

Chen, Jun ^{[1
,2
]}

Wang, Hai ^{[1
,2
]}

Wu, Gui ^{[1
,2
]}

Li, Bo ^{[1
,2
]}

Li, Qiang ^{[1
,2
]}

Huang, Zhifeng ^{[1
,2
]}

Qiu, Guixing ^{[1
,2
]}

Wu, Zhihong ^{[1
,2
,5
,6
]}

机构：

[1] Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Orthopaed Surg, 1 Shuaifuyuan, Beijing 100730, Peoples R China

[2] Chinese Acad Med Sci, 1 Shuaifuyuan, Beijing 100730, Peoples R China

[3] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China

[4] Peking Union Med Coll, Beijing 100050, Peoples R China

[5] Peking Union Med Coll Hosp, Peking Union Med Coll, Cent Lab, 1 Shuaifuyuan, Beijing 100730, Peoples R China

[6] Beijing Key Lab Genet Res Bone & Joint Dis, 1 Shuaifuyuan, Beijing 100730, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2016年 / 17卷 / 04期

基金：

中国国家自然科学基金;

关键词：

porous titanium; Gel/nHA; 3D micro-scaffolds; hybrid scaffold; osteogenesis; CALCIUM-PHOSPHATE; AUTOLOGOUS BONE; BIOMIMETIC MATERIALS; ILIAC CREST; IN-VIVO; HYDROXYAPATITE; GRAFT; FABRICATION; DEFECTS; GELATIN;

D O I：

10.3390/ijms17040575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porous titanium is a kind of promising material for bone substitution, while its bio-inert property results in demand of modifications to improve the osteointegration capacity. In this study, gelatin (Gel) and nano-hydroxyapatite (nHA) were used to construct 3D micro-scaffolds in the pores of porous titanium in the ratios of Gel: nHA = 1: 0, Gel: nHA = 1: 1, and Gel: nHA = 1: 3, respectively. Cell attachment and proliferation, and gene and protein expression levels of osteogenic markers were evaluated in MC3T3-E1 cells, followed by bone regeneration assessment in a rabbit radius defect model. All hybrid scaffolds with different composition ratio were found to have significant promotional effects in cell adhesion, proliferation and differentiation, in which the group with Gel: nHA = 1: 1 showed the best performance in vitro, as well as the most bone regeneration volume in vivo. This 3D micro-scaffolds modification may be an innovative method for porous titanium ornamentation and shows potential application values in clinic.

引用

页数：11

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