Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration

被引：20
作者：
Yin, Bo [1 ,2 ]
Ma, Pei [1 ,2 ,3 ,4 ]
Chen, Jun [1 ,2 ]
Wang, Hai [1 ,2 ]
Wu, Gui [1 ,2 ]
Li, Bo [1 ,2 ]
Li, Qiang [1 ,2 ]
Huang, Zhifeng [1 ,2 ]
Qiu, Guixing [1 ,2 ]
Wu, Zhihong [1 ,2 ,5 ,6 ]
机构：
[1] Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Orthopaed Surg, 1 Shuaifuyuan, Beijing 100730, Peoples R China
[2] Chinese Acad Med Sci, 1 Shuaifuyuan, Beijing 100730, Peoples R China
[3] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
[4] Peking Union Med Coll, Beijing 100050, Peoples R China
[5] Peking Union Med Coll Hosp, Peking Union Med Coll, Cent Lab, 1 Shuaifuyuan, Beijing 100730, Peoples R China
[6] Beijing Key Lab Genet Res Bone & Joint Dis, 1 Shuaifuyuan, Beijing 100730, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2016年 / 17卷 / 04期
基金：
中国国家自然科学基金;
关键词：
porous titanium; Gel/nHA; 3D micro-scaffolds; hybrid scaffold; osteogenesis; CALCIUM-PHOSPHATE; AUTOLOGOUS BONE; BIOMIMETIC MATERIALS; ILIAC CREST; IN-VIVO; HYDROXYAPATITE; GRAFT; FABRICATION; DEFECTS; GELATIN;
D O I：
10.3390/ijms17040575
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Porous titanium is a kind of promising material for bone substitution, while its bio-inert property results in demand of modifications to improve the osteointegration capacity. In this study, gelatin (Gel) and nano-hydroxyapatite (nHA) were used to construct 3D micro-scaffolds in the pores of porous titanium in the ratios of Gel: nHA = 1: 0, Gel: nHA = 1: 1, and Gel: nHA = 1: 3, respectively. Cell attachment and proliferation, and gene and protein expression levels of osteogenic markers were evaluated in MC3T3-E1 cells, followed by bone regeneration assessment in a rabbit radius defect model. All hybrid scaffolds with different composition ratio were found to have significant promotional effects in cell adhesion, proliferation and differentiation, in which the group with Gel: nHA = 1: 1 showed the best performance in vitro, as well as the most bone regeneration volume in vivo. This 3D micro-scaffolds modification may be an innovative method for porous titanium ornamentation and shows potential application values in clinic.
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页数：11
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