Far-field optical nanoscopy

被引:2238
作者
Hell, Stefan W. [1 ]
机构
[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, D-37070 Gottingen, Germany
[2] Deutsch Krebsforschungszentrum, German Canc Res Ctr, High Resolut Opt Microscopy Div, D-69120 Heidelberg, Germany
关键词
D O I
10.1126/science.1137395
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In 1873, Ernst Abbe discovered what was to become a well-known paradigm: the inability of a lens-based optical microscope to discern details that are closer together than half of the wavelength of light. However, for its most popular imaging mode, fluorescence microscopy, the diffraction barrier is crumbling. Here, I discuss the physical concepts that have pushed fluorescence microscopy to the nanoscale, once the prerogative of electron and scanning probe microscopes. Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
引用
收藏
页码:1153 / 1158
页数:6
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